Abstract
Two versatile Clostridium perfringens-Escherichia coli shuttle vectors were constructed. Each plasmid carried a single antibiotic resistance gene which was expressed in both organisms. The plasmid pJIR750 encoded resistance to chloramphenicol and pJIR751 encoded resistance to erythromycin. Each plasmid contained the pUC18-derived multiple cloning site and the lacZ' gene which enabled direct screening for recombinants in E. coli. These plasmids should prove invaluable for the genetic manipulation of C. perfringens.
| Original language | English |
|---|---|
| Pages (from-to) | 233-235 |
| Number of pages | 3 |
| Journal | Plasmid |
| Volume | 29 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Jan 1993 |