Clostridium perfringens-escherichia coli shuttle vectors that carry single antibiotic resistance determinants

Trudi L. Bannam, Julian I. Rood

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Two versatile Clostridium perfringens-Escherichia coli shuttle vectors were constructed. Each plasmid carried a single antibiotic resistance gene which was expressed in both organisms. The plasmid pJIR750 encoded resistance to chloramphenicol and pJIR751 encoded resistance to erythromycin. Each plasmid contained the pUC18-derived multiple cloning site and the lacZ' gene which enabled direct screening for recombinants in E. coli. These plasmids should prove invaluable for the genetic manipulation of C. perfringens.

Original languageEnglish
Pages (from-to)233-235
Number of pages3
Issue number3
Publication statusPublished - 1 Jan 1993

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