Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for multiple ligands (CcmL)

Mayra A. Machuca, Yu C. Liu, Simone A. Beckham, Anna Roujeinikova

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A periplasmic sensory domain of the Campylobacter jejuni chemoreceptor for multiple ligands (CcmL) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. A complete data set was collected to 1.3 Å resolution using cryocooling conditions and synchrotron radiation. The crystals belonged to space group P21, with unit-cell parameters a = 42.6, b = 138.0, c = 49.0 Å, β = 94.3 º .
Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalActa Crystallographica Section F: Structural Biology Communications
Issue numberPart 2
Publication statusPublished - 2015


  • campylobacter jejuni
  • chemotaxis
  • transducer-like proteins
  • methyl-accepting proteins

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