TY - JOUR
T1 - Cloning, purification and preliminary crystallographic analysis of the Bacillus subtilis GTPase YphC-GDP complex
AU - Xu, Ling
AU - Muench, Stephen P
AU - Roujeinikova, Anna
AU - Sedelnikova, Svetlana E
AU - Rice, David W
PY - 2006
Y1 - 2006
N2 - The Bacillus subtilis YphC gene encodes an essential GTPase thought to be involved in ribosome binding and whose protein product may represent a target for the development of a novel antibacterial agent. Sequence analysis reveals that YphC belongs to the EngA family of GTPases, which uniquely contain two adjacent GTP-binding domains. Crystals of a selenomethionine-incorporated YphC-GDP complex have been grown using the hanging-drop vapour-diffusion method and polyethylene glycol as a precipitating agent. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 62.71, b = 65.05, c = 110.61 angstroms, and have one molecule in the asymmetric unit. Data sets at three different wavelengths were collected on a single crystal to 2.5 angstroms resolution at the Daresbury SRS in order to solve the structure by MAD. Ultimately, analysis of YphC in complex with GDP may allow a greater understanding of the EngA family of essential GTPases.
AB - The Bacillus subtilis YphC gene encodes an essential GTPase thought to be involved in ribosome binding and whose protein product may represent a target for the development of a novel antibacterial agent. Sequence analysis reveals that YphC belongs to the EngA family of GTPases, which uniquely contain two adjacent GTP-binding domains. Crystals of a selenomethionine-incorporated YphC-GDP complex have been grown using the hanging-drop vapour-diffusion method and polyethylene glycol as a precipitating agent. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 62.71, b = 65.05, c = 110.61 angstroms, and have one molecule in the asymmetric unit. Data sets at three different wavelengths were collected on a single crystal to 2.5 angstroms resolution at the Daresbury SRS in order to solve the structure by MAD. Ultimately, analysis of YphC in complex with GDP may allow a greater understanding of the EngA family of essential GTPases.
UR - http://www.ncbi.nlm.nih.gov/pubmed/16682769
M3 - Article
SN - 1744-3091
VL - 62
SP - 435
EP - 437
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
IS - Pt 5
ER -