Cloning of sucrase genes from Streptococcus mutans in bacteriophage lambda

R. R.B. Russell, P. Morrissey, G. Dougan

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Chromosomal DNA from Streptococcus mutans strain Ingbritt (serotype c) was cloned into the bacteriophage λ replacement vector L47.1. The bank of recombinant phage was screened for the presence of plaques in which sucrose-hydrolysing (sucrase) activity was expressed. Two distinct sucrase-expressing recombinants were identified. In one type, designated scr, the product is an invertase-like enzyme. This enzyme was purified from lysates of recombinants and shown to have an apparent Mr of 59000. The second class of recombinants was found to express a glucosyltransferase, identical in size, as well as in enzymatic and antigenic properties, to the previously described product of gtfA.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalFEMS Microbiology Letters
Volume30
Issue number1-2
DOIs
Publication statusPublished - 1 Jan 1985
Externally publishedYes

Keywords

  • Genetic engineering
  • streptococci
  • sugar metabolism

Cite this