Chromosomal DNA from Streptococcus mutans strain Ingbritt (serotype c) was cloned into the bacteriophage λ replacement vector L47.1. The bank of recombinant phage was screened for the presence of plaques in which sucrose-hydrolysing (sucrase) activity was expressed. Two distinct sucrase-expressing recombinants were identified. In one type, designated scr, the product is an invertase-like enzyme. This enzyme was purified from lysates of recombinants and shown to have an apparent Mr of 59000. The second class of recombinants was found to express a glucosyltransferase, identical in size, as well as in enzymatic and antigenic properties, to the previously described product of gtfA.
|Number of pages||5|
|Journal||FEMS Microbiology Letters|
|Publication status||Published - 1 Jan 1985|
- Genetic engineering
- sugar metabolism