TY - JOUR
T1 - Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis
AU - Kefala, Georgia
AU - Janowski, Robert
AU - Panjikar, Santosh
AU - Mueller-Dieckmann, Christoph
AU - Weiss, Manfred S.
PY - 2005/7
Y1 - 2005/7
N2 - Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.
AB - Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.
UR - http://www.scopus.com/inward/record.url?scp=33744493119&partnerID=8YFLogxK
U2 - 10.1107/S174430910501938X
DO - 10.1107/S174430910501938X
M3 - Article
C2 - 16511139
AN - SCOPUS:33744493119
SN - 1744-3091
VL - 61
SP - 718
EP - 721
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 7
ER -
