Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis

Georgia Kefala; Robert Janowski; Santosh Panjikar; Christoph Mueller-Dieckmann; Manfred S. Weiss

doi:10.1107/S174430910501938X

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis

Georgia Kefala, Robert Janowski, Santosh Panjikar, Christoph Mueller-Dieckmann, Manfred S. Weiss

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.

Original language	English
Pages (from-to)	718-721
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	61
Issue number	7
DOIs	https://doi.org/10.1107/S174430910501938X
Publication status	Published - Jul 2005
Externally published	Yes

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10.1107/S174430910501938X

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Cite this

Kefala, G., Janowski, R., Panjikar, S., Mueller-Dieckmann, C., & Weiss, M. S. (2005). Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(7), 718-721. https://doi.org/10.1107/S174430910501938X

Kefala, Georgia ; Janowski, Robert ; Panjikar, Santosh ; Mueller-Dieckmann, Christoph ; Weiss, Manfred S. / Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2005 ; Vol. 61, No. 7. pp. 718-721.

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abstract = "Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.",

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Kefala, G, Janowski, R, Panjikar, S, Mueller-Dieckmann, C & Weiss, MS 2005, 'Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 61, no. 7, pp. 718-721. https://doi.org/10.1107/S174430910501938X

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis. / Kefala, Georgia; Janowski, Robert; Panjikar, Santosh; Mueller-Dieckmann, Christoph; Weiss, Manfred S.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 7, 07.2005, p. 718-721.

Research output: Contribution to journal › Article › Research › peer-review

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