Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis

Georgia Kefala, Robert Janowski, Santosh Panjikar, Christoph Mueller-Dieckmann, Manfred S. Weiss

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10 Citations (Scopus)

Abstract

Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.

Original languageEnglish
Pages (from-to)718-721
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number7
DOIs
Publication statusPublished - Jul 2005
Externally publishedYes

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