Mycobacterium species have developed numerous strategies to avoid the antimycobacterial actions of macrophages, enabling them to survive within the generally inhospitable environment of the cell. The recently identified MSMEG_5817 protein from M. smegmatis is highly conserved in Mycobacterium spp. and is required for bacterial survival in macrophages. Here, the cloning, expression, purification and crystallization of MSMEG_5817 is reported. Crystals of MSMEG_5817 were grown in 1.42 M Li2SO4, 0.1 M Tris-HCl pH 7.7, 0.1 M sodium citrate tribasic dihydrate. Native and multiple-wavelength anomalous dispersion (MAD) data sets have been collected and structure determination is in progress.
|Pages (from-to)||566 - 569|
|Number of pages||4|
|Journal||Acta Crystallographica. Section F: Structural Biology Communications|
|Publication status||Published - 2013|
Shahine, A. E., Chan, P. Y., Littler, D., Vivian, J., Brammananth, R., Crellin, P. K., Coppel, R. L., Rossjohn, J., & Beddoe, T. C. (2013). Cloning, expression, purification and preliminary X-ray diffraction studies of a mycobacterial protein implicated in bacterial survival in macrophages. Acta Crystallographica. Section F: Structural Biology Communications, 69(5), 566 - 569. https://doi.org/10.1107/S1744309113010427