Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

Dhanasekaran Sakthivel; Dene Littler; Adam E Shahine; Sally T Troy; Matthew David Johnson; Jamie Rossjohn; David M Piedrafita; Travis C Beddoe

doi:10.1107/S2053230X15010195

Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

Dhanasekaran Sakthivel, Dene Littler, Adam E Shahine, Sally T Troy, Matthew David Johnson, Jamie Rossjohn, David M Piedrafita, Travis C Beddoe

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.

Original language	English
Pages (from-to)	993-997
Number of pages	5
Journal	Acta Crystallographica Section F: Structural Biology Communications
Volume	71
Issue number	Pt 8
DOIs	https://doi.org/10.1107/S2053230X15010195
Publication status	Published - 2015

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10.1107/S2053230X15010195

Cite this

Sakthivel, D., Littler, D., Shahine, A. E., Troy, S. T., Johnson, M. D., Rossjohn, J., Piedrafita, D. M., & Beddoe, T. C. (2015). Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries). Acta Crystallographica Section F: Structural Biology Communications, 71(Pt 8), 993-997. https://doi.org/10.1107/S2053230X15010195

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abstract = "Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.",

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AU - Troy, Sally T

AU - Johnson, Matthew David

AU - Rossjohn, Jamie

AU - Piedrafita, David M

AU - Beddoe, Travis C

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