Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

Dhanasekaran Sakthivel, Dene Littler, Adam E Shahine, Sally T Troy, Matthew David Johnson, Jamie Rossjohn, David M Piedrafita, Travis C Beddoe

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.
Original languageEnglish
Pages (from-to)993-997
Number of pages5
JournalActa Crystallographica. Section F: Structural Biology Communications
Volume71
Issue numberPt 8
DOIs
Publication statusPublished - 2015

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