TY - JOUR
T1 - Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)
AU - Sakthivel, Dhanasekaran
AU - Littler, Dene
AU - Shahine, Adam E
AU - Troy, Sally T
AU - Johnson, Matthew David
AU - Rossjohn, Jamie
AU - Piedrafita, David M
AU - Beddoe, Travis C
PY - 2015
Y1 - 2015
N2 - Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.
AB - Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.
U2 - 10.1107/S2053230X15010195
DO - 10.1107/S2053230X15010195
M3 - Article
VL - 71
SP - 993
EP - 997
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
SN - 2053-230X
IS - Pt 8
ER -