Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.
|Number of pages||5|
|Journal||Acta Crystallographica. Section F: Structural Biology Communications|
|Issue number||Pt 8|
|Publication status||Published - 2015|
Sakthivel, D., Littler, D., Shahine, A. E., Troy, S. T., Johnson, M. D., Rossjohn, J., ... Beddoe, T. C. (2015). Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries). Acta Crystallographica. Section F: Structural Biology Communications, 71(Pt 8), 993-997. https://doi.org/10.1107/S2053230X15010195