Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

Dhanasekaran Sakthivel; Dene Littler; Adam E Shahine; Sally T Troy; Matthew David Johnson; Jamie Rossjohn; David M Piedrafita; Travis C Beddoe

doi:10.1107/S2053230X15010195

Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

Dhanasekaran Sakthivel, Dene Littler, Adam E Shahine, Sally T Troy, Matthew David Johnson, Jamie Rossjohn, David M Piedrafita, Travis C Beddoe

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.

Original language	English
Pages (from-to)	993-997
Number of pages	5
Journal	Acta Crystallographica Section F: Structural Biology Communications
Volume	71
Issue number	Pt 8
DOIs	https://doi.org/10.1107/S2053230X15010195
Publication status	Published - 2015

Access to Document

10.1107/S2053230X15010195

Cite this

Sakthivel, D., Littler, D., Shahine, A. E., Troy, S. T., Johnson, M. D., Rossjohn, J., Piedrafita, D. M., & Beddoe, T. C. (2015). Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries). Acta Crystallographica Section F: Structural Biology Communications, 71(Pt 8), 993-997. https://doi.org/10.1107/S2053230X15010195

Sakthivel, Dhanasekaran ; Littler, Dene ; Shahine, Adam E ; Troy, Sally T ; Johnson, Matthew David ; Rossjohn, Jamie ; Piedrafita, David M ; Beddoe, Travis C. / Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries). In: Acta Crystallographica Section F: Structural Biology Communications. 2015 ; Vol. 71, No. Pt 8. pp. 993-997.

@article{6981fc9a498d484ca55eebad46c22f60,

title = "Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)",

abstract = "Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.",

author = "Dhanasekaran Sakthivel and Dene Littler and Shahine, {Adam E} and Troy, {Sally T} and Johnson, {Matthew David} and Jamie Rossjohn and Piedrafita, {David M} and Beddoe, {Travis C}",

year = "2015",

doi = "10.1107/S2053230X15010195",

language = "English",

volume = "71",

pages = "993--997",

journal = "Acta Crystallographica Section F: Structural Biology Communications",

issn = "2053-230X",

publisher = "International Union of Crystallography (IUCr)",

number = "Pt 8",

}

Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries). / Sakthivel, Dhanasekaran; Littler, Dene ; Shahine, Adam E; Troy, Sally T; Johnson, Matthew David; Rossjohn, Jamie; Piedrafita, David M; Beddoe, Travis C.

In: Acta Crystallographica Section F: Structural Biology Communications, Vol. 71, No. Pt 8, 2015, p. 993-997.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

AU - Sakthivel, Dhanasekaran

AU - Littler, Dene

AU - Shahine, Adam E

AU - Troy, Sally T

AU - Johnson, Matthew David

AU - Rossjohn, Jamie

AU - Piedrafita, David M

AU - Beddoe, Travis C

PY - 2015

Y1 - 2015

N2 - Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.

AB - Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 A resolution are reported.

U2 - 10.1107/S2053230X15010195

DO - 10.1107/S2053230X15010195

M3 - Article

VL - 71

SP - 993

EP - 997

JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

SN - 2053-230X

IS - Pt 8

ER -