TY - JOUR
T1 - Cloning and sequence analysis of ermQ, the predominant macrolide- lincosamide-streptogramin B resistance gene in Clostridium perfringens
AU - Berryman, D. I.
AU - Lyristis, M.
AU - Rood, J. I.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - The erythromycin resistance determinant from Clostridium perfringens JIR100 has been cloned, sequenced, and shown to be expressed in Escherichia coli. An open reading frame with sequence similarity to erm genes from other bacteria was identified and designated the ermQ gene. On the basis of comparative sequence analysis, it was concluded that the ermQ gene represented a new Erm hybridization class, designated ErmQ. Genes belonging to the ErmQ class were found to be widespread in C. perfringens, since 30 of 38 macrolide-lincosamide-streptogramin B-resistant C. perfringens strains, from diverse sources, hybridized to an ermQ-specific gene probe. The ermQ gene therefore represents the most common erythromycin resistance determinant in C. perfringens.
AB - The erythromycin resistance determinant from Clostridium perfringens JIR100 has been cloned, sequenced, and shown to be expressed in Escherichia coli. An open reading frame with sequence similarity to erm genes from other bacteria was identified and designated the ermQ gene. On the basis of comparative sequence analysis, it was concluded that the ermQ gene represented a new Erm hybridization class, designated ErmQ. Genes belonging to the ErmQ class were found to be widespread in C. perfringens, since 30 of 38 macrolide-lincosamide-streptogramin B-resistant C. perfringens strains, from diverse sources, hybridized to an ermQ-specific gene probe. The ermQ gene therefore represents the most common erythromycin resistance determinant in C. perfringens.
UR - http://www.scopus.com/inward/record.url?scp=0028216557&partnerID=8YFLogxK
U2 - 10.1128/AAC.38.5.1041
DO - 10.1128/AAC.38.5.1041
M3 - Article
C2 - 8067735
AN - SCOPUS:0028216557
SN - 0066-4804
VL - 38
SP - 1041
EP - 1046
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 5
ER -