Projects per year
Abstract
Immediate early response (IER) 2 gene, a member of the IER family, is a gene of unknown function which is affected by external stimuli in the brain. In the present study, the full length sequence and localization of medaka (Oryzias latipes) ier2 was investigated in the brain to understand the functions of Ier2 in the future studies. The full length sequence of medaka ier2 was identified using a 3'-, 5'- rapid amplification of cDNA ends method, and distribution in the brain was identified using in situ hybridization. The identified full length ier2 mRNA consisted of 939 nucleotides spanning along 1 exon. The deduced amino acid sequence consisted of 171 amino acid residues which contains a highly conserved sequence, nuclear localization signal. ier2 mRNA was distributed in the telencephalon, midbrain and the hypothalamus. This highly conserved primary response gene Ier2 can be used to visualize and map functionally activated neuronal circuitry in the brain of medaka.
Original language | English |
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Pages (from-to) | 24-29 |
Number of pages | 6 |
Journal | Journal of Chemical Neuroanatomy |
Volume | 77 |
DOIs | |
Publication status | Published - Nov 2016 |
Keywords
- Full length sequence
- Ier2
- In situ hybridization
- Medaka
Projects
- 2 Finished
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Roles of Orphan Receptors (SREB2 and 3 ) in Neurodegeneration
Shogo, M.
1/05/13 → 30/04/15
Project: Research
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MicroRNA-218a as a Novel Regulator of Reproduction : Implication in Overcoming Reproductive Aging
Chourasia, D.
1/05/13 → 30/04/15
Project: Research