TY - JOUR
T1 - Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family
AU - Degli-Esposti, Mariapia A.
AU - Smolak, Pamela J.
AU - Walczak, Henning
AU - Waugh, Jennifer
AU - Huang, Chang Pin
AU - DuBose, Robert F.
AU - Goodwin, Raymond G.
AU - Smith, Craig A.
PY - 1997/10/6
Y1 - 1997/10/6
N2 - TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. Transient expression and quantitative binding studies show TRAIL-R3 to be a plasma membrane-bound protein capable of high affinity interaction with the TRAIL ligand. The TRAIL-R3 gene maps to human chromosome 8p22-21, clustered with the genes encoding two other TRAIL receptors. In contrast to TRAIL-R1 and -R2, this receptor shows restricted expression, with transcripts detectable only in peripheral blood lymphocytes and spleen. The structure of TRAIL-R3 is unique when compared to the other TRAIL receptors in that it lacks a cytoplasmic domain and appears to be glycosyl-phosphatidylinositol-linked. Moreover, unlike TRAIL-R1 and -R2, in a transient overexpression system TRAIL-R3 does not induce apoptosis.
AB - TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. Transient expression and quantitative binding studies show TRAIL-R3 to be a plasma membrane-bound protein capable of high affinity interaction with the TRAIL ligand. The TRAIL-R3 gene maps to human chromosome 8p22-21, clustered with the genes encoding two other TRAIL receptors. In contrast to TRAIL-R1 and -R2, this receptor shows restricted expression, with transcripts detectable only in peripheral blood lymphocytes and spleen. The structure of TRAIL-R3 is unique when compared to the other TRAIL receptors in that it lacks a cytoplasmic domain and appears to be glycosyl-phosphatidylinositol-linked. Moreover, unlike TRAIL-R1 and -R2, in a transient overexpression system TRAIL-R3 does not induce apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=0030869432&partnerID=8YFLogxK
U2 - 10.1084/jem.186.7.1165
DO - 10.1084/jem.186.7.1165
M3 - Article
C2 - 9314565
AN - SCOPUS:0030869432
SN - 0022-1007
VL - 186
SP - 1165
EP - 1170
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 7
ER -