TY - JOUR
T1 - Clinical enzymes inhibitory activities, antioxidant potential and phytochemical profile of Vernonia oligocephala (DC.) Sch.Bip. ex Walp roots
AU - Mahmood, Waqas
AU - Saleem, Hammad
AU - Shahid, Wardah
AU - Ahmad, Irshad
AU - Zengin, Gokhan
AU - Mahomoodally, Mohamad Fawzi
AU - Ashraf, M.
AU - Ahemad, Nafees
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/3
Y1 - 2019/3
N2 - We investigated into the different solvent extracts (methanol, dichloromethane, ethyl acetate, n-hexane and n-butanol) of Vernonia oligocephala (DC.) Sch.Bip. ex Walp roots for their phytochemical composition, antioxidant and enzyme inhibition potential. Phytochemical analysis showed the presence of flavonoids, saponins, terpenoids, and phenolics as secondary metabolites. Methanol extract exhibited the highest phenolic (113.11 mg GAE/g) and flavonoid (97.35 mg QE/g) contents as well as ferric reducing antioxidant power (71.92 GAE/g). The ethyl acetate extract exhibited maximum DPPH (IC 50 ; 39.11 μg/mL), total antioxidant capacity (73.07 mg GAE/g) and urease inhibition (IC 50 ; 55.89 μg/mL). The n-hexane fraction was most active against cholinesterases. All extracts showed least activity against tyrosinase and lipoxygenase (except butanol fraction, IC 50 : 132.2 μg/mL). This study showed that V. oligocephala extracts can be regarded as perspective material for isolating bioactive molecules.
AB - We investigated into the different solvent extracts (methanol, dichloromethane, ethyl acetate, n-hexane and n-butanol) of Vernonia oligocephala (DC.) Sch.Bip. ex Walp roots for their phytochemical composition, antioxidant and enzyme inhibition potential. Phytochemical analysis showed the presence of flavonoids, saponins, terpenoids, and phenolics as secondary metabolites. Methanol extract exhibited the highest phenolic (113.11 mg GAE/g) and flavonoid (97.35 mg QE/g) contents as well as ferric reducing antioxidant power (71.92 GAE/g). The ethyl acetate extract exhibited maximum DPPH (IC 50 ; 39.11 μg/mL), total antioxidant capacity (73.07 mg GAE/g) and urease inhibition (IC 50 ; 55.89 μg/mL). The n-hexane fraction was most active against cholinesterases. All extracts showed least activity against tyrosinase and lipoxygenase (except butanol fraction, IC 50 : 132.2 μg/mL). This study showed that V. oligocephala extracts can be regarded as perspective material for isolating bioactive molecules.
KW - Antioxidant
KW - Cholinesterases
KW - Lipoxygenase
KW - Phytochemical
KW - Tyrosinase
KW - Urease
UR - http://www.scopus.com/inward/record.url?scp=85061933609&partnerID=8YFLogxK
U2 - 10.1016/j.bcab.2019.101039
DO - 10.1016/j.bcab.2019.101039
M3 - Article
AN - SCOPUS:85061933609
VL - 18
JO - Biocatalysis and Agricultural Biotechnology
JF - Biocatalysis and Agricultural Biotechnology
SN - 1878-8181
M1 - 101039
ER -