Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3

D. M. Wall; X. F. Hu; G. Nadalin; J. R. Zalcberg; J. D. Parkin; R. Sparrow; M. Van der Weyden; I. C. Marschner

doi:10.1016/S0959-8049(05)80216-3

Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3

D. M. Wall, X. F. Hu, G. Nadalin, J. R. Zalcberg, J. D. Parkin, R. Sparrow, M. Van der Weyden, I. C. Marschner

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.

Original language	English
Pages (from-to)	1024-1027
Number of pages	4
Journal	European Journal of Cancer
Volume	29
Issue number	7
DOIs	https://doi.org/10.1016/S0959-8049(05)80216-3
Publication status	Published - 1 Jan 1993
Externally published	Yes

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title = "Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3",

abstract = "A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.",

author = "Wall, {D. M.} and Hu, {X. F.} and G. Nadalin and Zalcberg, {J. R.} and Parkin, {J. D.} and R. Sparrow and {Van der Weyden}, M. and Marschner, {I. C.}",

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doi = "10.1016/S0959-8049(05)80216-3",

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AU - Wall, D. M.

AU - Hu, X. F.

AU - Nadalin, G.

AU - Zalcberg, J. R.

AU - Parkin, J. D.

AU - Sparrow, R.

AU - Van der Weyden, M.

AU - Marschner, I. C.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.

AB - A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.

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JF - European Journal of Cancer

SN - 0959-8049

IS - 7

ER -

Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3

Abstract

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