Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy

Molin Wongwattanakul, Chariya Hahnvajanawong, Patcharaporn Tippayawat, Sirinart Chio-Srichan, Chanvit Leelayuwat, Temduang Limpaiboon, Patcharee Jearanaikoon, Philip Heraud

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cholangiocarcinoma (CCA), a cancer of bile duct epithelium, is a major health problem in Thailand especially in the northeast. Overall treatment outcomes have not shown much improvement because the disease is usually detected at an advanced stage and often shows chemotherapeutic resistance. High-throughput Fourier Transform Infrared (FTIR) microspectroscopy can be used for cell classification and has the potential to diagnose cancer and possibly predict chemo-response. This study was aimed to differentiate gemcitabine-sensitive and gemcitabine-resistant induction in two CCA cell lines (KKU-M139 and KKU-M214) and xenograft tissues using synchrotron-FTIR microspectroscopy. Partial Least Squares Discriminant Analysis (PLS-DA) could discriminate between chemo-sensitive and chemo-resistant cells in the FTIR fingerprint spectral region (1800–1000 cm–1) with more than 90% sensitivity and specificity. The chemo-resistant and chemo-sensitive phenotypes were different in protein (amide I, amide II), lipids (carbonyl group and CH3 deformation) and phosphodiester from nucleic acids. Additionally, spectra from xenograft tissues showed similar results to the cell line study with marked differences between chemo-resistant and chemo-sensitive CCA tissues, and PLS-DA could discriminate the chemotherapeutic response with 98% sensitivity and specificity. This is the first study to demonstrate the use of FTIR microspectroscopy to assess chemo-response both in vitro and in vivo.
Original languageEnglish
Pages (from-to)367-376
Number of pages10
JournalJournal of Biophotonics
Volume10
Issue number3
DOIs
Publication statusPublished - 2017

Keywords

  • synchrotron-FTIR
  • cholangiocarcinoma
  • chemo-resistance
  • cell lines
  • animal model

Cite this

Wongwattanakul, M., Hahnvajanawong, C., Tippayawat, P., Chio-Srichan, S., Leelayuwat, C., Limpaiboon, T., ... Heraud, P. (2017). Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy. Journal of Biophotonics, 10(3), 367-376. https://doi.org/10.1002/jbio.201500253
Wongwattanakul, Molin ; Hahnvajanawong, Chariya ; Tippayawat, Patcharaporn ; Chio-Srichan, Sirinart ; Leelayuwat, Chanvit ; Limpaiboon, Temduang ; Jearanaikoon, Patcharee ; Heraud, Philip. / Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy. In: Journal of Biophotonics. 2017 ; Vol. 10, No. 3. pp. 367-376.
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title = "Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy",
abstract = "Cholangiocarcinoma (CCA), a cancer of bile duct epithelium, is a major health problem in Thailand especially in the northeast. Overall treatment outcomes have not shown much improvement because the disease is usually detected at an advanced stage and often shows chemotherapeutic resistance. High-throughput Fourier Transform Infrared (FTIR) microspectroscopy can be used for cell classification and has the potential to diagnose cancer and possibly predict chemo-response. This study was aimed to differentiate gemcitabine-sensitive and gemcitabine-resistant induction in two CCA cell lines (KKU-M139 and KKU-M214) and xenograft tissues using synchrotron-FTIR microspectroscopy. Partial Least Squares Discriminant Analysis (PLS-DA) could discriminate between chemo-sensitive and chemo-resistant cells in the FTIR fingerprint spectral region (1800–1000 cm–1) with more than 90{\%} sensitivity and specificity. The chemo-resistant and chemo-sensitive phenotypes were different in protein (amide I, amide II), lipids (carbonyl group and CH3 deformation) and phosphodiester from nucleic acids. Additionally, spectra from xenograft tissues showed similar results to the cell line study with marked differences between chemo-resistant and chemo-sensitive CCA tissues, and PLS-DA could discriminate the chemotherapeutic response with 98{\%} sensitivity and specificity. This is the first study to demonstrate the use of FTIR microspectroscopy to assess chemo-response both in vitro and in vivo.",
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author = "Molin Wongwattanakul and Chariya Hahnvajanawong and Patcharaporn Tippayawat and Sirinart Chio-Srichan and Chanvit Leelayuwat and Temduang Limpaiboon and Patcharee Jearanaikoon and Philip Heraud",
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Wongwattanakul, M, Hahnvajanawong, C, Tippayawat, P, Chio-Srichan, S, Leelayuwat, C, Limpaiboon, T, Jearanaikoon, P & Heraud, P 2017, 'Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy' Journal of Biophotonics, vol. 10, no. 3, pp. 367-376. https://doi.org/10.1002/jbio.201500253

Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy. / Wongwattanakul, Molin; Hahnvajanawong, Chariya; Tippayawat, Patcharaporn; Chio-Srichan, Sirinart; Leelayuwat, Chanvit; Limpaiboon, Temduang; Jearanaikoon, Patcharee; Heraud, Philip.

In: Journal of Biophotonics, Vol. 10, No. 3, 2017, p. 367-376.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy

AU - Wongwattanakul, Molin

AU - Hahnvajanawong, Chariya

AU - Tippayawat, Patcharaporn

AU - Chio-Srichan, Sirinart

AU - Leelayuwat, Chanvit

AU - Limpaiboon, Temduang

AU - Jearanaikoon, Patcharee

AU - Heraud, Philip

PY - 2017

Y1 - 2017

N2 - Cholangiocarcinoma (CCA), a cancer of bile duct epithelium, is a major health problem in Thailand especially in the northeast. Overall treatment outcomes have not shown much improvement because the disease is usually detected at an advanced stage and often shows chemotherapeutic resistance. High-throughput Fourier Transform Infrared (FTIR) microspectroscopy can be used for cell classification and has the potential to diagnose cancer and possibly predict chemo-response. This study was aimed to differentiate gemcitabine-sensitive and gemcitabine-resistant induction in two CCA cell lines (KKU-M139 and KKU-M214) and xenograft tissues using synchrotron-FTIR microspectroscopy. Partial Least Squares Discriminant Analysis (PLS-DA) could discriminate between chemo-sensitive and chemo-resistant cells in the FTIR fingerprint spectral region (1800–1000 cm–1) with more than 90% sensitivity and specificity. The chemo-resistant and chemo-sensitive phenotypes were different in protein (amide I, amide II), lipids (carbonyl group and CH3 deformation) and phosphodiester from nucleic acids. Additionally, spectra from xenograft tissues showed similar results to the cell line study with marked differences between chemo-resistant and chemo-sensitive CCA tissues, and PLS-DA could discriminate the chemotherapeutic response with 98% sensitivity and specificity. This is the first study to demonstrate the use of FTIR microspectroscopy to assess chemo-response both in vitro and in vivo.

AB - Cholangiocarcinoma (CCA), a cancer of bile duct epithelium, is a major health problem in Thailand especially in the northeast. Overall treatment outcomes have not shown much improvement because the disease is usually detected at an advanced stage and often shows chemotherapeutic resistance. High-throughput Fourier Transform Infrared (FTIR) microspectroscopy can be used for cell classification and has the potential to diagnose cancer and possibly predict chemo-response. This study was aimed to differentiate gemcitabine-sensitive and gemcitabine-resistant induction in two CCA cell lines (KKU-M139 and KKU-M214) and xenograft tissues using synchrotron-FTIR microspectroscopy. Partial Least Squares Discriminant Analysis (PLS-DA) could discriminate between chemo-sensitive and chemo-resistant cells in the FTIR fingerprint spectral region (1800–1000 cm–1) with more than 90% sensitivity and specificity. The chemo-resistant and chemo-sensitive phenotypes were different in protein (amide I, amide II), lipids (carbonyl group and CH3 deformation) and phosphodiester from nucleic acids. Additionally, spectra from xenograft tissues showed similar results to the cell line study with marked differences between chemo-resistant and chemo-sensitive CCA tissues, and PLS-DA could discriminate the chemotherapeutic response with 98% sensitivity and specificity. This is the first study to demonstrate the use of FTIR microspectroscopy to assess chemo-response both in vitro and in vivo.

KW - synchrotron-FTIR

KW - cholangiocarcinoma

KW - chemo-resistance

KW - cell lines

KW - animal model

UR - http://www.ncbi.nlm.nih.gov/pubmed/26996159

U2 - 10.1002/jbio.201500253

DO - 10.1002/jbio.201500253

M3 - Article

VL - 10

SP - 367

EP - 376

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 3

ER -

Wongwattanakul M, Hahnvajanawong C, Tippayawat P, Chio-Srichan S, Leelayuwat C, Limpaiboon T et al. Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy. Journal of Biophotonics. 2017;10(3):367-376. https://doi.org/10.1002/jbio.201500253