Cis-regulatory changes at FLOWERING LOCUS T mediate natural variation in flowering reponses of Arabidopsis thaliana

Christopher Schwartz, Sureshkumar Balasubramanian, Norman Warthmann, Todd Michael, Janne Lempe, Sridevi Sureshkumar, Yasushi Kobayashi, Julin Maloof, Justin Borevitz, Joanne Chory, Detlef Weigel

Research output: Contribution to journalArticleResearchpeer-review

85 Citations (Scopus)

Abstract

Flowering time, a critical adaptive trait, is modulated by several environmental cues. These external signals converge oil a small set of genes that in turn mediate the flowering response. Mutant analysis and subsequent molecular studies have revealed that one of these integrator genes, FLOWERING LOCUS T responds to photoperiod and temperature cues, two environmental parameters that greatly influence flowering time. As the central player in the transition to flowering, the protein coding sequence of FT and its function are highly conserved across species. Using QTL mapping with a new advanced intercross-recombinant inbred line (AI-RIL) population, we show that a QTL tightly linked to 1,7 contributes to natural variation in the flowering response to the combined effects of photoperiod and ambient temperature. Using heterogeneous inbred families (HIF) and introgression lines, we fine map the QTL to a 6.7 kb fragment in the FT promoter. We confirm by quantitative complementation that FT has differential activity in the two parental strains. Further support for FT underlying the QTL comes from a new approach, quantitative knockdown with artificial microRNAs (amiRNAs). Consistent with the causal sequence polymorphism being ill the promoter, we find that the QTL affects FT expression. Taken together, these results indicate that allelic variation at pathway integrator genes Such as FT can underlie phenotypic variability and that this may be achieved through cis-regulatory changes.
Original languageEnglish
Pages (from-to)723 - 732
Number of pages10
JournalGenetics
Volume183
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

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