Circulating microRNAs, Vascular Risk, and Physical Activity in Spinal Cord-Injured Subjects

Layde R Paim, Roberto Schreiber, Guilherme de Rossi, Jose R Matos-Souza, Anselmo de A. Costa e Silva, Decio Roberto Calegari, Susan Cheng, Francine Coelho Marques, Andrei C. Sposito, Jose I. Gorla, Alberto Cliquet, Jr, Wilson Nadruz, JR

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

The aim of this study was to compare the expression of serum microRNAs (miRNAs) in individuals with spinal cord injury (SCI) (athletes [SCI-A] and sedentary [SCI-S]) and able-bodied (AB) individuals, and investigate the relationship of miRNAs with carotid intima-media thickness (cIMT) and serum oxidized LDL-cholesterol (oxLDL) among SCI subjects. Seventeen SCI-S, 23 SCI-A, and 22 AB males were evaluated by clinical and laboratory analysis, and had oxLDL and cIMT measured by enzyme-linked immunosorbent assay (ELISA) and ultrasonography, respectively. A total of 754 miRNAs were measured using a TaqMan OpenArray® Human MicroRNA system.

SCI-S subjects had higher cIMT and oxLDL than SCI-A and AB. Compared with AB, only one miRNA was differently expressed in both SCI-A and SCI-S individuals, whereas 25 miRNAs were differently expressed in SCI-S, but not in SCI-A. Of these 25 miRNAs, 22 showed different expression between SCI-S and SCI-A. Several miRNAs correlated with oxLDL and cIMT among all SCI individuals. Notably, miR-125b-5p, miR-146a-5p, miR-328-3p, miR-191-5p, miR-103a-3p, and miR-30b-5p correlated with both oxLDL and cIMT, and showed distinct expression between the SCI-A and SCI-S groups. Gene set enrichment analysis demonstrated that miRNAs related to cIMT and oxLDL may be involved in molecular pathways regulating vascular function and remodeling. In conclusion, this exploratory analysis suggests that variations in circulating miRNA expression in individuals with SCI compared with AB subjects are markedly attenuated by regular physical activity. Several miRNAs may be involved in physical activity-related improvements in vascular risk and remodeling among SCI individuals.
Original languageEnglish
Pages (from-to)845-852
Number of pages8
JournalJournal of Neurotrauma
Volume36
Issue number6
DOIs
Publication statusPublished - 2019

Keywords

  • cardiovascular risk
  • exercise
  • intima-media thickness
  • miRNA
  • spinal cord injury

Cite this

Paim, L. R., Schreiber, R., de Rossi, G., Matos-Souza, J. R., de A. Costa e Silva, A., Calegari, D. R., ... Nadruz, JR, W. (2019). Circulating microRNAs, Vascular Risk, and Physical Activity in Spinal Cord-Injured Subjects. Journal of Neurotrauma, 36(6), 845-852. https://doi.org/10.1089/neu.2018.5880
Paim, Layde R ; Schreiber, Roberto ; de Rossi, Guilherme ; Matos-Souza, Jose R ; de A. Costa e Silva, Anselmo ; Calegari, Decio Roberto ; Cheng, Susan ; Coelho Marques, Francine ; Sposito, Andrei C. ; Gorla, Jose I. ; Cliquet, Jr, Alberto ; Nadruz, JR, Wilson . / Circulating microRNAs, Vascular Risk, and Physical Activity in Spinal Cord-Injured Subjects. In: Journal of Neurotrauma. 2019 ; Vol. 36, No. 6. pp. 845-852.
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abstract = "The aim of this study was to compare the expression of serum microRNAs (miRNAs) in individuals with spinal cord injury (SCI) (athletes [SCI-A] and sedentary [SCI-S]) and able-bodied (AB) individuals, and investigate the relationship of miRNAs with carotid intima-media thickness (cIMT) and serum oxidized LDL-cholesterol (oxLDL) among SCI subjects. Seventeen SCI-S, 23 SCI-A, and 22 AB males were evaluated by clinical and laboratory analysis, and had oxLDL and cIMT measured by enzyme-linked immunosorbent assay (ELISA) and ultrasonography, respectively. A total of 754 miRNAs were measured using a TaqMan OpenArray{\circledR} Human MicroRNA system.SCI-S subjects had higher cIMT and oxLDL than SCI-A and AB. Compared with AB, only one miRNA was differently expressed in both SCI-A and SCI-S individuals, whereas 25 miRNAs were differently expressed in SCI-S, but not in SCI-A. Of these 25 miRNAs, 22 showed different expression between SCI-S and SCI-A. Several miRNAs correlated with oxLDL and cIMT among all SCI individuals. Notably, miR-125b-5p, miR-146a-5p, miR-328-3p, miR-191-5p, miR-103a-3p, and miR-30b-5p correlated with both oxLDL and cIMT, and showed distinct expression between the SCI-A and SCI-S groups. Gene set enrichment analysis demonstrated that miRNAs related to cIMT and oxLDL may be involved in molecular pathways regulating vascular function and remodeling. In conclusion, this exploratory analysis suggests that variations in circulating miRNA expression in individuals with SCI compared with AB subjects are markedly attenuated by regular physical activity. Several miRNAs may be involved in physical activity-related improvements in vascular risk and remodeling among SCI individuals.",
keywords = "cardiovascular risk, exercise, intima-media thickness, miRNA, spinal cord injury",
author = "Paim, {Layde R} and Roberto Schreiber and {de Rossi}, Guilherme and Matos-Souza, {Jose R} and {de A. Costa e Silva}, Anselmo and Calegari, {Decio Roberto} and Susan Cheng and {Coelho Marques}, Francine and Sposito, {Andrei C.} and Gorla, {Jose I.} and {Cliquet, Jr}, Alberto and {Nadruz, JR}, Wilson",
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Paim, LR, Schreiber, R, de Rossi, G, Matos-Souza, JR, de A. Costa e Silva, A, Calegari, DR, Cheng, S, Coelho Marques, F, Sposito, AC, Gorla, JI, Cliquet, Jr, A & Nadruz, JR, W 2019, 'Circulating microRNAs, Vascular Risk, and Physical Activity in Spinal Cord-Injured Subjects', Journal of Neurotrauma, vol. 36, no. 6, pp. 845-852. https://doi.org/10.1089/neu.2018.5880

Circulating microRNAs, Vascular Risk, and Physical Activity in Spinal Cord-Injured Subjects. / Paim, Layde R; Schreiber, Roberto; de Rossi, Guilherme; Matos-Souza, Jose R; de A. Costa e Silva, Anselmo; Calegari, Decio Roberto; Cheng, Susan; Coelho Marques, Francine; Sposito, Andrei C.; Gorla, Jose I. ; Cliquet, Jr, Alberto; Nadruz, JR, Wilson .

In: Journal of Neurotrauma, Vol. 36, No. 6, 2019, p. 845-852.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Circulating microRNAs, Vascular Risk, and Physical Activity in Spinal Cord-Injured Subjects

AU - Paim, Layde R

AU - Schreiber, Roberto

AU - de Rossi, Guilherme

AU - Matos-Souza, Jose R

AU - de A. Costa e Silva, Anselmo

AU - Calegari, Decio Roberto

AU - Cheng, Susan

AU - Coelho Marques, Francine

AU - Sposito, Andrei C.

AU - Gorla, Jose I.

AU - Cliquet, Jr, Alberto

AU - Nadruz, JR, Wilson

PY - 2019

Y1 - 2019

N2 - The aim of this study was to compare the expression of serum microRNAs (miRNAs) in individuals with spinal cord injury (SCI) (athletes [SCI-A] and sedentary [SCI-S]) and able-bodied (AB) individuals, and investigate the relationship of miRNAs with carotid intima-media thickness (cIMT) and serum oxidized LDL-cholesterol (oxLDL) among SCI subjects. Seventeen SCI-S, 23 SCI-A, and 22 AB males were evaluated by clinical and laboratory analysis, and had oxLDL and cIMT measured by enzyme-linked immunosorbent assay (ELISA) and ultrasonography, respectively. A total of 754 miRNAs were measured using a TaqMan OpenArray® Human MicroRNA system.SCI-S subjects had higher cIMT and oxLDL than SCI-A and AB. Compared with AB, only one miRNA was differently expressed in both SCI-A and SCI-S individuals, whereas 25 miRNAs were differently expressed in SCI-S, but not in SCI-A. Of these 25 miRNAs, 22 showed different expression between SCI-S and SCI-A. Several miRNAs correlated with oxLDL and cIMT among all SCI individuals. Notably, miR-125b-5p, miR-146a-5p, miR-328-3p, miR-191-5p, miR-103a-3p, and miR-30b-5p correlated with both oxLDL and cIMT, and showed distinct expression between the SCI-A and SCI-S groups. Gene set enrichment analysis demonstrated that miRNAs related to cIMT and oxLDL may be involved in molecular pathways regulating vascular function and remodeling. In conclusion, this exploratory analysis suggests that variations in circulating miRNA expression in individuals with SCI compared with AB subjects are markedly attenuated by regular physical activity. Several miRNAs may be involved in physical activity-related improvements in vascular risk and remodeling among SCI individuals.

AB - The aim of this study was to compare the expression of serum microRNAs (miRNAs) in individuals with spinal cord injury (SCI) (athletes [SCI-A] and sedentary [SCI-S]) and able-bodied (AB) individuals, and investigate the relationship of miRNAs with carotid intima-media thickness (cIMT) and serum oxidized LDL-cholesterol (oxLDL) among SCI subjects. Seventeen SCI-S, 23 SCI-A, and 22 AB males were evaluated by clinical and laboratory analysis, and had oxLDL and cIMT measured by enzyme-linked immunosorbent assay (ELISA) and ultrasonography, respectively. A total of 754 miRNAs were measured using a TaqMan OpenArray® Human MicroRNA system.SCI-S subjects had higher cIMT and oxLDL than SCI-A and AB. Compared with AB, only one miRNA was differently expressed in both SCI-A and SCI-S individuals, whereas 25 miRNAs were differently expressed in SCI-S, but not in SCI-A. Of these 25 miRNAs, 22 showed different expression between SCI-S and SCI-A. Several miRNAs correlated with oxLDL and cIMT among all SCI individuals. Notably, miR-125b-5p, miR-146a-5p, miR-328-3p, miR-191-5p, miR-103a-3p, and miR-30b-5p correlated with both oxLDL and cIMT, and showed distinct expression between the SCI-A and SCI-S groups. Gene set enrichment analysis demonstrated that miRNAs related to cIMT and oxLDL may be involved in molecular pathways regulating vascular function and remodeling. In conclusion, this exploratory analysis suggests that variations in circulating miRNA expression in individuals with SCI compared with AB subjects are markedly attenuated by regular physical activity. Several miRNAs may be involved in physical activity-related improvements in vascular risk and remodeling among SCI individuals.

KW - cardiovascular risk

KW - exercise

KW - intima-media thickness

KW - miRNA

KW - spinal cord injury

U2 - 10.1089/neu.2018.5880

DO - 10.1089/neu.2018.5880

M3 - Article

VL - 36

SP - 845

EP - 852

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

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ER -

Paim LR, Schreiber R, de Rossi G, Matos-Souza JR, de A. Costa e Silva A, Calegari DR et al. Circulating microRNAs, Vascular Risk, and Physical Activity in Spinal Cord-Injured Subjects. Journal of Neurotrauma. 2019;36(6):845-852. https://doi.org/10.1089/neu.2018.5880