TY - JOUR
T1 - Chronic ocular hypertension induced by circumlimbal suture in rats
AU - Liu, Edward Hsin-Hua
AU - Bui, Bang V
AU - Nguyen, Christine T O
AU - Kezic, Jelena M
AU - Vingrys, Algis J
AU - He, Zheng
PY - 2015
Y1 - 2015
N2 - PURPOSE: To induce chronic intraocular pressure (IOP) elevation in rat eyes by circumlimbal suture. METHODS: Anesthetized (isoflurane) Long-Evans rats underwent unilateral circumlimbal suture implantation while the fellow eyes served as untreated controls (n=15). A sham group (n=8) received the same procedure except the suture was loosely tied. IOP, electroretinography (ERG) and optical coherence tomography (OCT) were monitored for 15 weeks, after which retinal histology and immunofluorescence staining for glial fibrillary acidic protein (GFAP) and ionized calcium binding adapter molecule-1 (Iba-1) were undertaken. RESULTS: Both IOP and ERG remained unaltered in the sham and all control eyes over 15 weeks. In the ocular hypertensive eye, IOP spiked from 17+/-1 to 58+/-3 mmHg immediately after suture application, recovering to 32+/-2 mmHg by 24 hours, and remained elevated by 7-10 mmHg above baseline for 15 weeks. At week 2, there was a small reduction of ERG components involving the photoreceptor a-wave, bipolar cell b-wave, and ganglion cell mediated Scotopic Threshold Response (pSTR). The reduction in a- and b-wave remained stable, while the pSTR became more affected from week 8 onwards (p
AB - PURPOSE: To induce chronic intraocular pressure (IOP) elevation in rat eyes by circumlimbal suture. METHODS: Anesthetized (isoflurane) Long-Evans rats underwent unilateral circumlimbal suture implantation while the fellow eyes served as untreated controls (n=15). A sham group (n=8) received the same procedure except the suture was loosely tied. IOP, electroretinography (ERG) and optical coherence tomography (OCT) were monitored for 15 weeks, after which retinal histology and immunofluorescence staining for glial fibrillary acidic protein (GFAP) and ionized calcium binding adapter molecule-1 (Iba-1) were undertaken. RESULTS: Both IOP and ERG remained unaltered in the sham and all control eyes over 15 weeks. In the ocular hypertensive eye, IOP spiked from 17+/-1 to 58+/-3 mmHg immediately after suture application, recovering to 32+/-2 mmHg by 24 hours, and remained elevated by 7-10 mmHg above baseline for 15 weeks. At week 2, there was a small reduction of ERG components involving the photoreceptor a-wave, bipolar cell b-wave, and ganglion cell mediated Scotopic Threshold Response (pSTR). The reduction in a- and b-wave remained stable, while the pSTR became more affected from week 8 onwards (p
UR - http://iovs.arvojournals.org/article.aspx?articleid=2290246
U2 - 10.1167/iovs.14-16009
DO - 10.1167/iovs.14-16009
M3 - Article
VL - 56
SP - 2811
EP - 2820
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 1552-5783
IS - 5
ER -