Chromosome- and plasmid-mediated gentamicin resistance in Staphylococcus aureus encoded by Tn4001

M. T. Gillespie, B. R. Lyon, L. J. Messerotti, R. A. Skurray

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DNA sequences corresponding to the 4.7-kb gentamicin, tobramycin and kanamycin resistance (Gm(r)Tm(r)Km(r)) transposon Tn4001 have been detected on a series of nine structurally-related plasmids that mediate this phenotype in Australian isolates of Staphylococcus aureus. Tn4001 sequences have also been demonstrated on the chromosomes of Gm(r)Tm(r)Km(r) isolates that do not possess these plasmids, and the exhibited diversity of chromosomal sites occupied by this element implies that Tn4001 has transposed to the chromosome on numerous occasions in vivo. These results suggest that the rapid emergence of nosoco1ial Gm(r)Tm(r)Km(r) S. aureus in the early 1980s may have been the result of the transposition of Tn4001 from a chromosomal site to a readily disseminated plasmid.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalJournal of Medical Microbiology
Issue number2
Publication statusPublished - 1 Jan 1987

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