Chromosomal location of TOL plasmid DNA in Pseudomonas putida

M. I. Sinclair; P. C. Maxwell; B. R. Lyon; B. W. Holloway

doi:10.1128/jb.168.3.1302-1308.1986

Chromosomal location of TOL plasmid DNA in Pseudomonas putida

M. I. Sinclair, P. C. Maxwell, B. R. Lyon, B. W. Holloway

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Abstract

The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18 hybrids has shown that the TOL segment is almost identical to the same region found in the TOL plasmid pWW0.

Original language	English
Pages (from-to)	1302-1308
Number of pages	7
Journal	Journal of Bacteriology
Volume	168
Issue number	3
DOIs	https://doi.org/10.1128/jb.168.3.1302-1308.1986
Publication status	Published - 1 Jan 1986

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10.1128/jb.168.3.1302-1308.1986

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abstract = "The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18 hybrids has shown that the TOL segment is almost identical to the same region found in the TOL plasmid pWW0.",

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AU - Sinclair, M. I.

AU - Maxwell, P. C.

AU - Lyon, B. R.

AU - Holloway, B. W.

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N2 - The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18 hybrids has shown that the TOL segment is almost identical to the same region found in the TOL plasmid pWW0.

AB - The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18 hybrids has shown that the TOL segment is almost identical to the same region found in the TOL plasmid pWW0.

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Chromosomal location of TOL plasmid DNA in Pseudomonas putida

Abstract

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