Chromosomal insertion of TOL transposons in Pseudomonas aeruginosa PAO

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Abstract

Insertions of the TOL plasmid transposons Tn4651 and Tn4653 into the Pseudomonas aeruginosa PAO chromosome were isolated by a temperature selection technique. The locations and orientations of 16 insertions were determined by pulsed field gel electrophoresis and Southern hybridization with genomic and TOL DNA probes. All insertions occurred within a 334 kb region of the chromosome (representing less than 6% of the genome) with nine of the inserts clustered within a 10 kb area. Each transposon was able to insert in either orientation. An internal duplication of the 39 kb excisable region of pWW0 was seen in two independent insertions.

Original languageEnglish
Pages (from-to)1111-1120
Number of pages10
JournalJournal of General Microbiology
Volume137
Issue number5
DOIs
Publication statusPublished - 1 Jan 1991

Cite this

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title = "Chromosomal insertion of TOL transposons in Pseudomonas aeruginosa PAO",
abstract = "Insertions of the TOL plasmid transposons Tn4651 and Tn4653 into the Pseudomonas aeruginosa PAO chromosome were isolated by a temperature selection technique. The locations and orientations of 16 insertions were determined by pulsed field gel electrophoresis and Southern hybridization with genomic and TOL DNA probes. All insertions occurred within a 334 kb region of the chromosome (representing less than 6{\%} of the genome) with nine of the inserts clustered within a 10 kb area. Each transposon was able to insert in either orientation. An internal duplication of the 39 kb excisable region of pWW0 was seen in two independent insertions.",
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Chromosomal insertion of TOL transposons in Pseudomonas aeruginosa PAO. / Sinclair, M. I.; Holloway, B. W.

In: Journal of General Microbiology, Vol. 137, No. 5, 01.01.1991, p. 1111-1120.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Sinclair, M. I.

AU - Holloway, B. W.

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AB - Insertions of the TOL plasmid transposons Tn4651 and Tn4653 into the Pseudomonas aeruginosa PAO chromosome were isolated by a temperature selection technique. The locations and orientations of 16 insertions were determined by pulsed field gel electrophoresis and Southern hybridization with genomic and TOL DNA probes. All insertions occurred within a 334 kb region of the chromosome (representing less than 6% of the genome) with nine of the inserts clustered within a 10 kb area. Each transposon was able to insert in either orientation. An internal duplication of the 39 kb excisable region of pWW0 was seen in two independent insertions.

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