TY - JOUR
T1 - Chitinophaga extrema sp. Nov., isolated from subsurface soil and leaf litter in a tropical peat swamp forest
AU - Goh, Calvin Bok Sun
AU - Wong, Li Wen
AU - Parimannan, Sivachandran
AU - Rajandas, Heera
AU - Loke, Stella
AU - Croft, Lawrence
AU - Yule, Catherine Mary
AU - Pasbakhsh, Pooria
AU - Lee, Sui Mae
AU - Tan, Joash Ban Lee
PY - 2020
Y1 - 2020
N2 - A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1T was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a Koompassia malaccensis tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39′ 04.7′ N 101° 17′ 43.7′′ E). Phylogenetic analyses based on the full 16S rRNA sequence revealed that strain Mgbs1T belongs to the genus Chitinophaga with the greatest sequence similarity to Chitinophaga terrae KP01T (97.65%), Chitinophaga jiangningensis DSM27406T (97.58%), and Chitinophaga dinghuensis DHOC24T (97.17%). The major fatty acids of strain Mgbs1T (>10%) are iso-C15:0, C16:1 ω5c and iso-C17:0 3-OH while the predominant respiratory quinone is menaquinone-7. Strain Mgbs1T has a complete genome size of 8.03 Mb, with a G+C content of 48.5 mol%. The DNA–DNA hybridization (DDH) score between strain Mgbs1T and C. jiangningensis DSM27406T was 15.9%, while in silico DDH values of strain Mgbs1T against C. dinghuensis DHOC24T and C. terrae KP01T were 20.0 and 19.10% respectively. Concurrently, Average Nucleotide Identity (ANI) scores between strain Mgbs1T against all three reference strains are 73.2%. Based on the phenotypic, chemotaxonomic, and phylogenetic consensus, strain Mgbs1T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga extrema sp. nov. is proposed (=DSM 108835T=JCM 33276T).
AB - A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1T was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a Koompassia malaccensis tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39′ 04.7′ N 101° 17′ 43.7′′ E). Phylogenetic analyses based on the full 16S rRNA sequence revealed that strain Mgbs1T belongs to the genus Chitinophaga with the greatest sequence similarity to Chitinophaga terrae KP01T (97.65%), Chitinophaga jiangningensis DSM27406T (97.58%), and Chitinophaga dinghuensis DHOC24T (97.17%). The major fatty acids of strain Mgbs1T (>10%) are iso-C15:0, C16:1 ω5c and iso-C17:0 3-OH while the predominant respiratory quinone is menaquinone-7. Strain Mgbs1T has a complete genome size of 8.03 Mb, with a G+C content of 48.5 mol%. The DNA–DNA hybridization (DDH) score between strain Mgbs1T and C. jiangningensis DSM27406T was 15.9%, while in silico DDH values of strain Mgbs1T against C. dinghuensis DHOC24T and C. terrae KP01T were 20.0 and 19.10% respectively. Concurrently, Average Nucleotide Identity (ANI) scores between strain Mgbs1T against all three reference strains are 73.2%. Based on the phenotypic, chemotaxonomic, and phylogenetic consensus, strain Mgbs1T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga extrema sp. nov. is proposed (=DSM 108835T=JCM 33276T).
KW - Chitinophaga extrema
KW - Koompassia malaccensis
KW - Malaysia
KW - Phylogeny
KW - Taxonomy
KW - Tropical peat swamp
UR - http://www.scopus.com/inward/record.url?scp=85098241175&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004539
DO - 10.1099/ijsem.0.004539
M3 - Article
C2 - 33146596
AN - SCOPUS:85098241175
SN - 1466-5026
VL - 70
SP - 6355
EP - 6363
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 12
ER -