TY - JOUR
T1 - Chemometric optimisation of enzymatic hydrolysis of beechwood xylan to target desired xylooligosaccharides
AU - Díaz-Arenas, Gloria L.
AU - Lebanov, Leo
AU - Sanz Rodríguez, Estrella
AU - Sadiq, M. Munir
AU - Paull, Brett
AU - Garnier, Gil
AU - Tanner, Joanne
N1 - Funding Information:
The authors wish to express their gratitude to ARC Industrial Transformation Research Hub for Processing Advanced Lignocellulosics Products (PALs) grant for this research (Grant: ARC IH170100020). The funding from the Australian Government Research Training Program (RTP) Scholarship to G. L. D?az-Arenas is greatly appreciated. The authors are also grateful to the Department of Chemical and Biological Engineering of Monash University and the School of Natural Sciences of the University of Tasmania. The authors acknowledge Thermo Fisher Scientific for placement of the IC-QqQ-MS instrument within the University of Tasmania to undertake this work. The authors would also like to acknowledge Dr M. Naseri for the provision of technical support.
Funding Information:
The authors wish to express their gratitude to ARC Industrial Transformation Research Hub for Processing Advanced Lignocellulosics Products (PALs) grant for this research (Grant: ARC IH170100020). The funding from the Australian Government Research Training Program (RTP) Scholarship to G. L. Díaz-Arenas is greatly appreciated. The authors are also grateful to the Department of Chemical and Biological Engineering of Monash University and the School of Natural Sciences of the University of Tasmania. The authors acknowledge Thermo Fisher Scientific for placement of the IC-QqQ-MS instrument within the University of Tasmania to undertake this work. The authors would also like to acknowledge Dr M. Naseri for the provision of technical support.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/5
Y1 - 2022/5
N2 - Generation of specific xylooligosaccharides (XOS) is attractive to the pharmaceutical and food industries due to the importance of their structure upon their application. This study used chemometrics to develop a comprehensive computational modelling set to predict the parameters maximising the generation of the desired XOS during enzymatic hydrolysis. The evaluated parameters included pH, temperature, substrate concentration, enzyme dosage and reaction time. A Box-Behnken design was combined with response surface methodology to develop the models. High-performance anion-exchange chromatography coupled with triple-quadrupole mass spectrometry (HPAEC-QqQ-MS) allowed the identification of 22 XOS within beechwood xylan hydrolysates. These data were used to validate the developed models and demonstrated their accuracy in predicting the parameters maximising the generation of the desired XOS. The maximum yields for X2-X6 were 314.2 ± 1.2, 76.6 ± 4.5, 38.4 ± 0.4, 17.8 ± 0.7, and 5.3 ± 0.2 mg/g xylan, respectively. These values map closely to the model predicted values 311.7, 92.6, 43.0, 16.3, and 4.9 mg/g xylan, respectively.
AB - Generation of specific xylooligosaccharides (XOS) is attractive to the pharmaceutical and food industries due to the importance of their structure upon their application. This study used chemometrics to develop a comprehensive computational modelling set to predict the parameters maximising the generation of the desired XOS during enzymatic hydrolysis. The evaluated parameters included pH, temperature, substrate concentration, enzyme dosage and reaction time. A Box-Behnken design was combined with response surface methodology to develop the models. High-performance anion-exchange chromatography coupled with triple-quadrupole mass spectrometry (HPAEC-QqQ-MS) allowed the identification of 22 XOS within beechwood xylan hydrolysates. These data were used to validate the developed models and demonstrated their accuracy in predicting the parameters maximising the generation of the desired XOS. The maximum yields for X2-X6 were 314.2 ± 1.2, 76.6 ± 4.5, 38.4 ± 0.4, 17.8 ± 0.7, and 5.3 ± 0.2 mg/g xylan, respectively. These values map closely to the model predicted values 311.7, 92.6, 43.0, 16.3, and 4.9 mg/g xylan, respectively.
KW - Endoxylanases
KW - Enzymatic hydrolysis
KW - Mass Spectrometry
KW - Xylan, Xylooligosaccharides
UR - http://www.scopus.com/inward/record.url?scp=85127290631&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2022.127041
DO - 10.1016/j.biortech.2022.127041
M3 - Article
C2 - 35318144
AN - SCOPUS:85127290631
VL - 352
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
M1 - 127041
ER -