Projects per year
Abstract
Generation of specific xylooligosaccharides (XOS) is attractive to the pharmaceutical and food industries due to the importance of their structure upon their application. This study used chemometrics to develop a comprehensive computational modelling set to predict the parameters maximising the generation of the desired XOS during enzymatic hydrolysis. The evaluated parameters included pH, temperature, substrate concentration, enzyme dosage and reaction time. A Box-Behnken design was combined with response surface methodology to develop the models. High-performance anion-exchange chromatography coupled with triple-quadrupole mass spectrometry (HPAEC-QqQ-MS) allowed the identification of 22 XOS within beechwood xylan hydrolysates. These data were used to validate the developed models and demonstrated their accuracy in predicting the parameters maximising the generation of the desired XOS. The maximum yields for X2-X6 were 314.2 ± 1.2, 76.6 ± 4.5, 38.4 ± 0.4, 17.8 ± 0.7, and 5.3 ± 0.2 mg/g xylan, respectively. These values map closely to the model predicted values 311.7, 92.6, 43.0, 16.3, and 4.9 mg/g xylan, respectively.
Original language | English |
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Article number | 127041 |
Number of pages | 9 |
Journal | Bioresource Technology |
Volume | 352 |
DOIs | |
Publication status | Published - May 2022 |
Keywords
- Endoxylanases
- Enzymatic hydrolysis
- Mass Spectrometry
- Xylan, Xylooligosaccharides
Projects
- 1 Finished
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ARC Research Hub for Processing Lignocellulosics into High Value Products
Garnier, G. (Primary Chief Investigator (PCI)), Batchelor, W. (Chief Investigator (CI)), Simon, G. (Chief Investigator (CI)), Haritos, V. (Chief Investigator (CI)), Patti, A. (Chief Investigator (CI)), Saito, K. (Chief Investigator (CI)), Griesser, H. (Chief Investigator (CI)), Paull, B. (Chief Investigator (CI)), Tanner, J. (Chief Investigator (CI)), Spinnler, H. (Partner Investigator (PI)), Allais, F. (Partner Investigator (PI)), Richardson, D. (Partner Investigator (PI)), Mackay, A. (Partner Investigator (PI)), Carter, S. (Partner Investigator (PI)), Faltas, R. (Partner Investigator (PI)), Edye, L. (Partner Investigator (PI)), Hendriks, D. (Partner Investigator (PI)), Karmakar, N. (Chief Investigator (CI)), Bhattacharya, S. (Chief Investigator (CI)) & Hawe, N. (Project Manager)
Monash University – Internal University Contribution, Monash University – Internal Faculty Contribution, Monash University – Internal Department Contribution, Paper Australia Pty Ltd, Leaf Resources Pty Ltd, Department of State Growth (Tasmania), University of Tasmania, University of South Australia, Agro Biotechnologies Industrielles, Visy Industries Australia Pty Ltd (trading as Visy Industries), Norske Skog Paper Mills (Australia) Pty Ltd, Orora Limited (trading as AMCOR Australia)
10/01/18 → 31/12/24
Project: Research