Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications

Rania A. Hashad; Ritu Singla; Sukhvir Kaur Bhangu; Edwina Jap; Haiyan Zhu; Anton Y. Peleg; Luke Blakeway; Christoph E. Hagemeyer; Francesca Cavalieri; Muthupandian Ashokkumar; Karen Alt

doi:10.1016/j.ultsonch.2022.106183

Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications

Rania A. Hashad, Ritu Singla, Sukhvir Kaur Bhangu, Edwina Jap, Haiyan Zhu, Anton Y. Peleg, Luke Blakeway, Christoph E. Hagemeyer, Francesca Cavalieri, Muthupandian Ashokkumar, Karen Alt

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

Nisin, a peptide used as a natural food preservative, is employed in this work for the development of a novel nanocarrier system. Stable and uniform nisin-shelled nanoemulsions (NSNE) with a diameter of 100 ± 20 nm were successfully prepared using 20 kHz flow-through ultrasonication technique. The NSNE showed limited toxicity, high bactericidal activity and high drug loading capacity (EE 65 % w/w). In addition, the nisin shell was exploited for the site-specific attachment of a recombinantly produced cancer targeting ligand (αHER2_LPETG IgG). Employing a unique two phases (bio-click) approach which involved both Sortase A mediated Azide Bioconjugation (SMAB) and Strain Promoted Azide Alkyne Cycloaddition (SPAAC) reactions, targeted NSNE (NSNE_DOX-αHER2 IgG) were successfully assembled and loaded with the chemotherapeutic drug Doxorubicin (DOX). Finally, NSNE_DOX-αHER2 IgG showed cancer-specific binding and augmented cytotoxicity to HER2 expressing tumour cells.

Original language	English
Article number	106183
Number of pages	8
Journal	Ultrasonics Sonochemistry
Volume	90
DOIs	https://doi.org/10.1016/j.ultsonch.2022.106183
Publication status	Published - Nov 2022

Keywords

Drug delivery
Nanoemulsion
Nisin
Ultrasonication

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ultsonch.2022.106183Licence: CC BY-NC

Cite this

Hashad, R. A., Singla, R., Kaur Bhangu, S., Jap, E., Zhu, H., Peleg, A. Y., Blakeway, L., Hagemeyer, C. E., Cavalieri, F., Ashokkumar, M., & Alt, K. (2022). Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications. Ultrasonics Sonochemistry, 90, Article 106183. https://doi.org/10.1016/j.ultsonch.2022.106183

@article{c4d051d0b9cf418e92d40aad41b117a1,

title = "Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications",

abstract = "Nisin, a peptide used as a natural food preservative, is employed in this work for the development of a novel nanocarrier system. Stable and uniform nisin-shelled nanoemulsions (NSNE) with a diameter of 100 ± 20 nm were successfully prepared using 20 kHz flow-through ultrasonication technique. The NSNE showed limited toxicity, high bactericidal activity and high drug loading capacity (EE 65 \% w/w). In addition, the nisin shell was exploited for the site-specific attachment of a recombinantly produced cancer targeting ligand (αHER2LPETG IgG). Employing a unique two phases (bio-click) approach which involved both Sortase A mediated Azide Bioconjugation (SMAB) and Strain Promoted Azide Alkyne Cycloaddition (SPAAC) reactions, targeted NSNE (NSNEDOX-αHER2 IgG) were successfully assembled and loaded with the chemotherapeutic drug Doxorubicin (DOX). Finally, NSNEDOX-αHER2 IgG showed cancer-specific binding and augmented cytotoxicity to HER2 expressing tumour cells.",

keywords = "Drug delivery, Nanoemulsion, Nisin, Ultrasonication",

author = "Hashad, \{Rania A.\} and Ritu Singla and \{Kaur Bhangu\}, Sukhvir and Edwina Jap and Haiyan Zhu and Peleg, \{Anton Y.\} and Luke Blakeway and Hagemeyer, \{Christoph E.\} and Francesca Cavalieri and Muthupandian Ashokkumar and Karen Alt",

note = "Funding Information: The authors would like to thank Shweta Jagdale for technical assistance. This work is supported by the National Health and Medical Research Council of Australia (Career Development Fellowship GNT1140465 to K.A., Senior Research Fellowship to C.E.H. GNT1154270). The authors also wish to thank AMREP Flow Cytometry Core Facility (Eva Orlowski-Oliver and Dr Magdaline Costa) and Binghe Wang (Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia 30303 United States) for kindly providing the click-activated smart florescent (SFC) probe. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = nov,

doi = "10.1016/j.ultsonch.2022.106183",

language = "English",

volume = "90",

journal = "Ultrasonics Sonochemistry",

issn = "1350-4177",

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T2 - Novel targeted drug-nanocarriers for cancer applications

AU - Hashad, Rania A.

AU - Singla, Ritu

AU - Kaur Bhangu, Sukhvir

AU - Jap, Edwina

AU - Zhu, Haiyan

AU - Peleg, Anton Y.

AU - Blakeway, Luke

AU - Hagemeyer, Christoph E.

AU - Cavalieri, Francesca

AU - Ashokkumar, Muthupandian

AU - Alt, Karen

N1 - Funding Information: The authors would like to thank Shweta Jagdale for technical assistance. This work is supported by the National Health and Medical Research Council of Australia (Career Development Fellowship GNT1140465 to K.A., Senior Research Fellowship to C.E.H. GNT1154270). The authors also wish to thank AMREP Flow Cytometry Core Facility (Eva Orlowski-Oliver and Dr Magdaline Costa) and Binghe Wang (Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia 30303 United States) for kindly providing the click-activated smart florescent (SFC) probe. Publisher Copyright: © 2022 The Author(s)

PY - 2022/11

Y1 - 2022/11

N2 - Nisin, a peptide used as a natural food preservative, is employed in this work for the development of a novel nanocarrier system. Stable and uniform nisin-shelled nanoemulsions (NSNE) with a diameter of 100 ± 20 nm were successfully prepared using 20 kHz flow-through ultrasonication technique. The NSNE showed limited toxicity, high bactericidal activity and high drug loading capacity (EE 65 % w/w). In addition, the nisin shell was exploited for the site-specific attachment of a recombinantly produced cancer targeting ligand (αHER2LPETG IgG). Employing a unique two phases (bio-click) approach which involved both Sortase A mediated Azide Bioconjugation (SMAB) and Strain Promoted Azide Alkyne Cycloaddition (SPAAC) reactions, targeted NSNE (NSNEDOX-αHER2 IgG) were successfully assembled and loaded with the chemotherapeutic drug Doxorubicin (DOX). Finally, NSNEDOX-αHER2 IgG showed cancer-specific binding and augmented cytotoxicity to HER2 expressing tumour cells.

AB - Nisin, a peptide used as a natural food preservative, is employed in this work for the development of a novel nanocarrier system. Stable and uniform nisin-shelled nanoemulsions (NSNE) with a diameter of 100 ± 20 nm were successfully prepared using 20 kHz flow-through ultrasonication technique. The NSNE showed limited toxicity, high bactericidal activity and high drug loading capacity (EE 65 % w/w). In addition, the nisin shell was exploited for the site-specific attachment of a recombinantly produced cancer targeting ligand (αHER2LPETG IgG). Employing a unique two phases (bio-click) approach which involved both Sortase A mediated Azide Bioconjugation (SMAB) and Strain Promoted Azide Alkyne Cycloaddition (SPAAC) reactions, targeted NSNE (NSNEDOX-αHER2 IgG) were successfully assembled and loaded with the chemotherapeutic drug Doxorubicin (DOX). Finally, NSNEDOX-αHER2 IgG showed cancer-specific binding and augmented cytotoxicity to HER2 expressing tumour cells.

KW - Drug delivery

KW - Nanoemulsion

KW - Nisin

KW - Ultrasonication

UR - https://www.scopus.com/pages/publications/85139250622

U2 - 10.1016/j.ultsonch.2022.106183

DO - 10.1016/j.ultsonch.2022.106183

M3 - Article

C2 - 36201933

AN - SCOPUS:85139250622

SN - 1350-4177

VL - 90

JO - Ultrasonics Sonochemistry

JF - Ultrasonics Sonochemistry

M1 - 106183

ER -

Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Corrigendum to “Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications” [Ultrason. Sonochem. 90 (2022) 106183] (Ultrasonics Sonochemistry (2022) 90, (S1350417722002796), (10.1016/j.ultsonch.2022.106183))

Smart Targeted Nanoparticles for Diagnosis and Therapy

Novel targeted Onco-theranostic nanoparticles for personalised therapy and real-time monitoring

Cite this

Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Research output

Corrigendum to “Chemoenzymatic surface decoration of Nisin-shelled nanoemulsions: Novel targeted drug-nanocarriers for cancer applications” [Ultrason. Sonochem. 90 (2022) 106183] (Ultrasonics Sonochemistry (2022) 90, (S1350417722002796), (10.1016/j.ultsonch.2022.106183))

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Smart Targeted Nanoparticles for Diagnosis and Therapy

Novel targeted Onco-theranostic nanoparticles for personalised therapy and real-time monitoring

Cite this