Chemo-enzymatic synthesis and RAFT polymerization of 6-O-methacryloyl mannose: A suitable glycopolymer for binding to the tetrameric lectin concanavalin A?

Anthony M. Granville, Damien Quémener, Thomas P. Davis, Christopher Barner-Kowollik, Martina H. Stenzel

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44 Citations (Scopus)

Abstract

The chemo-enzymatic synthesis of 6-O-methacryloyl mannose (MaM) glycomonomer was successfully performed for the first time. Subsequent aqueous RAFT polymerization of the monomer yielded well-defined, linear poly(6-O-methacryloyl mannose) (PMaM) glycopolymers without the need for protecting and deprotecting group chemistry. As well as investigating the RAFT polymerization kinetics of this monomer using various initial monomer to chain transfer agent concentration ratios, the protein binding ability of the generated glycopolymer was tested using concanavalin A, a known mannose-residue binding lectin.

Original languageEnglish
Pages (from-to)81-89
Number of pages9
JournalMacromolecular Symposia
Volume255
DOIs
Publication statusPublished - 22 Oct 2007
Externally publishedYes

Keywords

  • Glycopolymer
  • Protein binding
  • RAFT polymerization
  • Reversible addition-fragmentation chain transfer polymerization

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