Chemical probes reveal the timing of early chlorination in vancomycin biosynthesis

Daniel J. Leng; Anja Greule; Max J. Cryle; Manuela Tosin

doi:10.1039/d0cc07421g

Chemical probes reveal the timing of early chlorination in vancomycin biosynthesis

Daniel J. Leng
, Anja Greule
, Max J. Cryle
, Manuela Tosin

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

Glycopeptides such as vancomycin are antibiotics of last resort whose biosynthetic pathways still hold undefined details. Chemical probes were used to capture biosynthetic intermediates generated in the nonribosomal peptide formation of vancomycinin vivo. The putative intercepted intermediates were characterisedviaHR-LC-MS². These species provided insights into the timing of the first chlorination of the peptide backbone by the halogenase VhaA: this holds significant interest for enzyme engineering towards the making of novel glycopeptides.

Original language	English
Pages (from-to)	2293-2296
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	18
DOIs	https://doi.org/10.1039/d0cc07421g
Publication status	Published - 4 Mar 2021

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10.1039/d0cc07421gLicence: CC BY

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abstract = "Glycopeptides such as vancomycin are antibiotics of last resort whose biosynthetic pathways still hold undefined details. Chemical probes were used to capture biosynthetic intermediates generated in the nonribosomal peptide formation of vancomycinin vivo. The putative intercepted intermediates were characterisedviaHR-LC-MS2. These species provided insights into the timing of the first chlorination of the peptide backbone by the halogenase VhaA: this holds significant interest for enzyme engineering towards the making of novel glycopeptides.",

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AU - Leng, Daniel J.

AU - Greule, Anja

AU - Cryle, Max J.

AU - Tosin, Manuela

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Y1 - 2021/3/4

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AB - Glycopeptides such as vancomycin are antibiotics of last resort whose biosynthetic pathways still hold undefined details. Chemical probes were used to capture biosynthetic intermediates generated in the nonribosomal peptide formation of vancomycinin vivo. The putative intercepted intermediates were characterisedviaHR-LC-MS2. These species provided insights into the timing of the first chlorination of the peptide backbone by the halogenase VhaA: this holds significant interest for enzyme engineering towards the making of novel glycopeptides.

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VL - 57

SP - 2293

EP - 2296

JO - Chemical Communications

JF - Chemical Communications

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ER -

Chemical probes reveal the timing of early chlorination in vancomycin biosynthesis

Abstract

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ARC Centre of Excellence for Innovations in Peptide and Protein Science

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Chemical probes reveal the timing of early chlorination in vancomycin biosynthesis

Abstract

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ARC Centre of Excellence for Innovations in Peptide and Protein Science

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