Chemical and biomolecule patterning on 2D surfaces using atmospheric pressure microcavity plasma array devices

Sameer A. Al-Bataineh, Endre J. Szili, Gilles Desmet, Paul Ruschitzka, Philipp J. Gruner, Craig Priest, Nicolas H. Voelcker, David A. Steele, Robert David Short, Hans Joerg Griesser

Research output: Chapter in Book/Report/Conference proceedingConference PaperOtherpeer-review

1 Citation (Scopus)


This paper presents a method for chemical and biomolecule patterning on planar (2D) surfaces using atmospheric pressure microplasmas. Spatially controlled surface modification is important for the development of emerging technologies such as microfluidic lab-on-a-chip devices, biosensors and other diagnostics tools. A non-fouling layer of poly(N-isopropylacrylamide) aldehyde (pNIPAM-ald) polymer, grafted onto heptylamine plasma polymer (HApp) modified silicon substrates, was used to achieve this goal. The non-fouling behaviour of the pNIPAM-ald coating was investigated at a temperature below its lower critical solution temperature (LCST) using human serum albumin (HSA). XPS and ToF-SIMS were used to characterise the plasma polymer coating and its subsequent modification with pNIPAM-ald before and after HSA adsorption. A 7 x 7 microcavity plasma array device (each cavity had a 250 μm diameter and was separated by 500 μm) was used for microplasma patterning. In a non-contact mode, helium microplasma treatment of the pNIPAM-ald coating was carried out for 60 s. The polymer coating was removed from regions directly exposed to microplasma cavities, as shown by ToF-SIMS. Microplasma treated regions were able to support the adsorption of fluorescently-labelled streptavidin whereas the rest of the coating was still non-fouling. This approach therefore resulted in spatially separated areas of immobilised protein.

Original languageEnglish
Title of host publicationSmart Nano-Micro Materials and Devices
Publication statusPublished - 2011
Externally publishedYes
EventSPIE Smart Nano + Micro Materials and Devices 2011 - Swinburne University of Technology, Melbourne, Australia
Duration: 4 Dec 20117 Dec 2011


ConferenceSPIE Smart Nano + Micro Materials and Devices 2011
Internet address


  • Fouling and non-fouling surfaces
  • Microarray
  • Microplasma
  • Protein
  • Surface patterning
  • ToF-SIMS
  • XPS

Cite this

Al-Bataineh, S. A., Szili, E. J., Desmet, G., Ruschitzka, P., Gruner, P. J., Priest, C., Voelcker, N. H., Steele, D. A., Short, R. D., & Griesser, H. J. (2011). Chemical and biomolecule patterning on 2D surfaces using atmospheric pressure microcavity plasma array devices. In Smart Nano-Micro Materials and Devices (Vol. 8204). [82043H]