TY - JOUR
T1 - Charge Has a Marked Influence on Hyperbranched Polymer Nanoparticle Association in Whole Human Blood
AU - Glass, Joshua Julian
AU - Chen, Liyu
AU - Alcantara, Sheilajen
AU - Crampin, Edmund J.
AU - Thurecht, Kristofer J.
AU - De Rose, Robert
AU - Kent, Stephen J.
PY - 2017/6/20
Y1 - 2017/6/20
N2 - In this study, we synthesize charge-varied hyperbranched polymers (HBPs) and demonstrate surface charge as a key parameter directing their association with specific human blood cell types. Using fresh human blood, we investigate the association of 5 nm HBPs with six white blood cell populations in their natural milieu by flow cytometry. While most cell types associate with cationic HBPs at 4 °C, at 37 °C phagocytic cells display similar (monocyte, dendritic cell) or greater (granulocyte) association with anionic HBPs compared to cationic HBPs. Neutral HBPs display remarkable stealth properties. Notably, these charge-association patterns are not solely defined by the plasma protein corona and are material and/or size dependent. As HBPs progress toward clinical use as imaging and drug delivery agents, the ability to engineer HBPs with defined biological properties is increasingly important. This knowledge can be used in the rational design of HBPs for more effective delivery to desired cell targets.
AB - In this study, we synthesize charge-varied hyperbranched polymers (HBPs) and demonstrate surface charge as a key parameter directing their association with specific human blood cell types. Using fresh human blood, we investigate the association of 5 nm HBPs with six white blood cell populations in their natural milieu by flow cytometry. While most cell types associate with cationic HBPs at 4 °C, at 37 °C phagocytic cells display similar (monocyte, dendritic cell) or greater (granulocyte) association with anionic HBPs compared to cationic HBPs. Neutral HBPs display remarkable stealth properties. Notably, these charge-association patterns are not solely defined by the plasma protein corona and are material and/or size dependent. As HBPs progress toward clinical use as imaging and drug delivery agents, the ability to engineer HBPs with defined biological properties is increasingly important. This knowledge can be used in the rational design of HBPs for more effective delivery to desired cell targets.
UR - https://www.scopus.com/pages/publications/85021077378
U2 - 10.1021/acsmacrolett.7b00229
DO - 10.1021/acsmacrolett.7b00229
M3 - Article
AN - SCOPUS:85021077378
SN - 2161-1653
VL - 6
SP - 586
EP - 592
JO - ACS Macro Letters
JF - ACS Macro Letters
IS - 6
ER -