Characterizing the disruption of HEK-293 cell membrane in AFM-based indentation using energy limiter method

Tianyao Shen, Bijan Shirinzadeh, Yongmin Zhong, Julian Smith

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

Abstract

The disruption of the cell membrane is an essential stage in adherent cell microinjection. With the assistant of atomic force microscopy system, the interaction force between the cell and the AFM probe during the indentation can be measured. Mechanical response of adherent cells in AFM-based indentation were explained by different models. However, the disruption of the cell membrane has not been theoretically explained. In this study, an energy limiter method is introduced to the continuum model of the adherent cells. The strain energy density function in the continuum model is modified by involving the energy limiter. As a result, on the computational nominal stress-indentation distance curve, a global maximum value of the nominal stress on the contact boundary between the cell membrane and the AFM probe can be found. Therefore, the location of the global maximum value can be used to characterize the disruption of the cell membrane. Indentation was conducted on the human kidney embryos 293 cells, and the performance of the energy limiter method in characterizing the disruption of the cell membrane was evaluated, which shows higher consistency than the performance of the experimental criterion. These results represent a feasible approach to understand the mechanism of the disruption of the cell membrane in microinjection.

Original languageEnglish
Title of host publication2019 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)
EditorsMiao Yu, Zhankun Weng
PublisherIEEE, Institute of Electrical and Electronics Engineers
Pages354-359
Number of pages6
ISBN (Electronic)9781728102054
DOIs
Publication statusPublished - 2020
EventInternational Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (M-NANO) 2019 - Zhenjiang, China
Duration: 4 Aug 20198 Aug 2019
Conference number: 9th

Publication series

Name2019 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2019 - Proceedings

Conference

ConferenceInternational Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (M-NANO) 2019
Abbreviated title3M-NANO 2019
CountryChina
CityZhenjiang
Period4/08/198/08/19

Keywords

  • Atomic force microscopy
  • Cell membrane
  • Energy limiter

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