Characterization of two TCR transgenic mouse lines specific for herpes simplex virus

Scott N. Mueller, William R. Heath, Julie D. Mclain, Francis R. Carbone, Claerwen M. Jones

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122 Citations (Scopus)

Abstract

To better understand the T cell-mediated processes involved in the immune response to herpes simplex virus type 1 (HSV-1) infection, two HSV-specific T cell receptor (TCR) transgenic mouse lines were produced. These mice (gBT-I.1 and gBT-I.3) are MHC class I-restricted and specific for the immunodominant peptide from HSV glycoprotein B (gB), gB498-505. Although derived from the same clone, the mice differ in the chromosomal location of the TCR transgenes and show marked differences in TCR α/β expression on both CD4+ and CD8+ cells in the thymus. Despite this, peripheral CD8+ T cells from both mice express equally high levels of the transgenic TCR and bind the KbgB498-505 tetramer to the same degree. In concordance with this, both were shown to respond equally well in vitro upon stimulation with the gB498-505 peptide or HSV-infected cells. These data show that selection of broadly equivalent peripheral T-cell subsets can occur in the presence of distinctly different thymic T-cell subsets.

Original languageEnglish
Pages (from-to)156-163
Number of pages8
JournalImmunology and Cell Biology
Volume80
Issue number2
DOIs
Publication statusPublished - 22 Apr 2002
Externally publishedYes

Keywords

  • CD8 T lymphocyte
  • Cytotoxic T lymphocyte
  • Herpes simplex virus
  • Major histocompatibility complex class I
  • T cell receptor
  • Thymus
  • Transgenic T cell receptor

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