Characterization of Tn3000, a transposon responsible for blaNDM-1 dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

Juliana Coutinho Campos, Maria José Félix Da Silva, Paulo Roberto Nascimento Dos Santos, Elaine Menezes Barros, Mayne De Oliveira Pereira, Bruna Mara Silva Seco, Cibele Massotti Magagnin, Leonardo Kalab Leiroz, Théo Gremen Mimary De Oliveira, Célio De Faria-Júnior, Louise Teixeira Cerdeira, Afonso Luís Barth, Suely Carlos Ferreira Sampaio, Alexandre Prehn Zavascki, Laurent Poirel, Jorge Luiz Mello Sampaio

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Abstract

In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world.

Original languageEnglish
Pages (from-to)7387-7395
Number of pages9
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number12
DOIs
Publication statusPublished - 1 Dec 2015
Externally publishedYes

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