Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: Implications for stem cell biology and tissue repair

Ting Yi Wang, Kiara A F Bruggeman, Rebecca K. Sheean, Bradley J. Turner, David R. Nisbet, Clare L. Parish

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

Background: Tethering proteins onto bioengineered scaffolds enables longterm delivery, however protein stability, release kinetics, and functionality over time remains unknown. Results: Tethered proteins remain stable and functional for several months, capable of activating intracellular signaling pathways and influencing cell fate. Conclusion: Tethered proteins are stable and functional long-term. Significance: Such knowledge may have implications for promoting tissue repair.

Original languageEnglish
Pages (from-to)15044-15051
Number of pages8
JournalThe Journal of Biological Chemistry
Volume289
Issue number21
DOIs
Publication statusPublished - 2014
Externally publishedYes

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