Characterization of the N-methyltransferase activities of the multifunctional polypeptide cyclosporin synthetase

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Abstract

This study examines the functions of these activities and demonstrates a critical role for N-methylation in cyclosporin biosynthesis and maintenance of the biologically active cyclosporin conformation. The structural requirements for the AdoMet binding to CySyn were defined. N-methylation of specific amide positions in the cyclosporin backbone is critical for the complete assembly and cyclization of the cyclosporin peptide. A maximum of two desmethyl positions is tolerated before peptide assembly stalls. Subinhibitory concentrations of AdoMet analogs directed peptide assembly towards cyclosporins with less than seven N-methylated amide bonds. Molecular modeling and nuclear magnetic resonance analysis indicate that N-methylation of specific amide bond positions in the cyclosporin backbone is mandatory for the formation of a product-like conformation and recognition by the acceptor site of the downstream peptide bond forming C-domain.
Original languageEnglish
Pages (from-to)464 - 475
Number of pages12
JournalChemistry and Biology
Volume18
Issue number4
DOIs
Publication statusPublished - 2011

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