Characterization of the mouse thrombomodulin gene and functional analysis of the 5′ flanking region in F9 teratocarcinoma cells

Peter Niforas, Georgina M. Sanderson, Catherina H. Bird, Phillip Bird

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)


The mouse thrombomodulin (TM) gene was examined and shown to be a single copy gene lacking introns. Two different clones each containing the entire mouse TM gene were isolated and the nucleotide sequence of a 1.4 kb fragment comprising the 5′ untranslated region and 1.2 kb of flanking sequences was determined. The transcriptional initiation site was located 30 bp downstream from a classical TATA motif within this fragment. This site was used in BALB/c 3T3 cells constitutively expressing TM, and when TM expression was induced in F9 teratocarcinoma cells in response to retinoic acid (RA) and dibutyryl cAMP (dbcAMP). A reporter construct consisting of the 1.4 kb fragment fused to the chloramphenicol acetyl transferase (CAT) gene was used to examine promoter function in F9 cells. CAT activity was induced on exposure to RA and dbcAMP and mimicked the pattern of expression of the endogenous TM gene. Induction of CAT activity did not depend on a sequence resembling a palindromic retinoic acid/thyroid hormone response element. We conclude that the 1.4 kb fragment contains the mouse TM promoter together with elements that control the induction of TM expression in differentiating F9 cells.

Original languageEnglish
Pages (from-to)179-187
Number of pages9
JournalBBA - Gene Structure and Expression
Issue number2
Publication statusPublished - 28 May 1993
Externally publishedYes


  • (Mouse)
  • F9 cell
  • Gene structure
  • Nucleic acid sequence
  • Promoter region
  • Thrombomodulin

Cite this