Characterization of the gut microbiota of Papua New Guineans using reverse transcription quantitative PCR

Andrew R. Greenhill, Hirokazu Tsuji, Kiyohito Ogata, Kazumi Natsuhara, Ayako Morita, Kevin Soli, Jo Ann Larkins, Kiyoshi Tadokoro, Shingo Odani, Jun Baba, Yuichi Naito, Eriko Tomitsuka, Koji Nomoto, Peter M. Siba, Paul F Horwood, Masahiro Umezaki

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

There has been considerable interest in composition of gut microbiota in recent years, leading to a better understanding of the role the gut microbiota plays in health and disease. Most studies have been limited in their geographical and socioeconomic diversity to high-income settings, and have been conducted using small sample sizes. To date, few analyses have been conducted in low-income settings, where a better understanding of the gut microbiome could lead to the greatest return in terms of health benefits. Here, we have used quantitative real-time polymerase chain reaction targeting dominant and sub-dominant groups of microorganisms associated with human gut microbiome in 115 people living a subsistence lifestyle in rural areas of Papua New Guinea. Quantification of Clostridium coccoides group, C. leptum subgroup, C. perfringens, Bacteroides fragilis group, Bifidobacterium, Atopobium cluster, Prevotella, Enterobacteriaceae, Enterococcus, Staphylococcus, and Lactobacillus spp. was conducted. Principle coordinates analysis (PCoA) revealed two dimensions with Prevotella, clostridia, Atopobium, Enterobacteriaceae, Enterococcus and Staphylococcus grouping in one dimension, while B. fragilis, Bifidobacterium and Lactobacillus grouping in the second dimension. Highland people had higher numbers of most groups of bacteria detected, and this is likely a key factor for the differences revealed by PCoA between highland and lowland study participants. Age and sex were not major determinants in microbial population composition. The study demonstrates a gut microbial composition with some similarities to those observed in other low-income settings where traditional diets are consumed, which have previously been suggested to favor energy extraction from a carbohydrate rich diet.

Original languageEnglish
Article numbere0117427
Number of pages15
JournalPLoS ONE
Volume10
Issue number2
DOIs
Publication statusPublished - 6 Feb 2015
Externally publishedYes

Keywords

  • prevotella
  • bacteria
  • gut bacteria
  • diet
  • lactobacillus
  • microbiome
  • adolescents
  • bacteroides

Cite this