Characterization of the antigen processing machinery and endogenous peptide presentation of a Bat MHC class I molecule

James W. Wynne, Amanda P. Woon, Nadine L. Dudek, Nathan P. Croft, Justin H. J. Ng, Michelle L. Baker, Lin-Fa Wang, Anthony W. Purcell

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Abstract

Bats are a major reservoir of emerging and re-emerging infectious diseases, including severe acute respiratory syndrome–like coronaviruses, henipaviruses, and Ebola virus. Although highly pathogenic to their spillover hosts, bats harbor these viruses, and a large number of other viruses, with little or no clinical signs of disease. How bats asymptomatically coexist with these viruses is unknown. In particular, little is known about bat adaptive immunity, and the presence of functional MHC molecules is mostly inferred from recently described genomes. In this study, we used an affinity purification/mass spectrometry approach to demonstrate that a bat MHC class I molecule, Ptal-N*01:01, binds antigenic peptides and associates with peptide-loading complex components. We identified several bat MHC class I–binding partners, including calnexin, calreticulin, protein disulfide isomerase A3, tapasin, TAP1, and TAP2. Additionally, endogenous peptide ligands isolated from Ptal-N*01:01 displayed a relatively broad length distribution and an unusual preference for a C-terminal proline residue. Finally, we demonstrate that this preference for C-terminal proline residues was observed in Hendra virus–derived peptides presented by Ptal-N*01:01 on the surface of infected cells. To our knowledge, this is the first study to identify endogenous and viral MHC class I ligands for any bat species and, as such, provides an important avenue for monitoring and development of vaccines against major bat-borne viruses both in the reservoir and spillover hosts. Additionally, it will provide a foundation to understand the role of adaptive immunity in bat antiviral responses.
Original languageEnglish
Pages (from-to)4468-4476
Number of pages9
JournalJournal of Immunology
Volume196
Issue number11
DOIs
Publication statusPublished - 1 Jun 2016

Cite this

Wynne, James W. ; Woon, Amanda P. ; Dudek, Nadine L. ; Croft, Nathan P. ; Ng, Justin H. J. ; Baker, Michelle L. ; Wang, Lin-Fa ; Purcell, Anthony W. / Characterization of the antigen processing machinery and endogenous peptide presentation of a Bat MHC class I molecule. In: Journal of Immunology. 2016 ; Vol. 196, No. 11. pp. 4468-4476.
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Characterization of the antigen processing machinery and endogenous peptide presentation of a Bat MHC class I molecule. / Wynne, James W.; Woon, Amanda P.; Dudek, Nadine L.; Croft, Nathan P.; Ng, Justin H. J.; Baker, Michelle L.; Wang, Lin-Fa; Purcell, Anthony W.

In: Journal of Immunology, Vol. 196, No. 11, 01.06.2016, p. 4468-4476.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Characterization of the antigen processing machinery and endogenous peptide presentation of a Bat MHC class I molecule

AU - Wynne, James W.

AU - Woon, Amanda P.

AU - Dudek, Nadine L.

AU - Croft, Nathan P.

AU - Ng, Justin H. J.

AU - Baker, Michelle L.

AU - Wang, Lin-Fa

AU - Purcell, Anthony W.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Bats are a major reservoir of emerging and re-emerging infectious diseases, including severe acute respiratory syndrome–like coronaviruses, henipaviruses, and Ebola virus. Although highly pathogenic to their spillover hosts, bats harbor these viruses, and a large number of other viruses, with little or no clinical signs of disease. How bats asymptomatically coexist with these viruses is unknown. In particular, little is known about bat adaptive immunity, and the presence of functional MHC molecules is mostly inferred from recently described genomes. In this study, we used an affinity purification/mass spectrometry approach to demonstrate that a bat MHC class I molecule, Ptal-N*01:01, binds antigenic peptides and associates with peptide-loading complex components. We identified several bat MHC class I–binding partners, including calnexin, calreticulin, protein disulfide isomerase A3, tapasin, TAP1, and TAP2. Additionally, endogenous peptide ligands isolated from Ptal-N*01:01 displayed a relatively broad length distribution and an unusual preference for a C-terminal proline residue. Finally, we demonstrate that this preference for C-terminal proline residues was observed in Hendra virus–derived peptides presented by Ptal-N*01:01 on the surface of infected cells. To our knowledge, this is the first study to identify endogenous and viral MHC class I ligands for any bat species and, as such, provides an important avenue for monitoring and development of vaccines against major bat-borne viruses both in the reservoir and spillover hosts. Additionally, it will provide a foundation to understand the role of adaptive immunity in bat antiviral responses.

AB - Bats are a major reservoir of emerging and re-emerging infectious diseases, including severe acute respiratory syndrome–like coronaviruses, henipaviruses, and Ebola virus. Although highly pathogenic to their spillover hosts, bats harbor these viruses, and a large number of other viruses, with little or no clinical signs of disease. How bats asymptomatically coexist with these viruses is unknown. In particular, little is known about bat adaptive immunity, and the presence of functional MHC molecules is mostly inferred from recently described genomes. In this study, we used an affinity purification/mass spectrometry approach to demonstrate that a bat MHC class I molecule, Ptal-N*01:01, binds antigenic peptides and associates with peptide-loading complex components. We identified several bat MHC class I–binding partners, including calnexin, calreticulin, protein disulfide isomerase A3, tapasin, TAP1, and TAP2. Additionally, endogenous peptide ligands isolated from Ptal-N*01:01 displayed a relatively broad length distribution and an unusual preference for a C-terminal proline residue. Finally, we demonstrate that this preference for C-terminal proline residues was observed in Hendra virus–derived peptides presented by Ptal-N*01:01 on the surface of infected cells. To our knowledge, this is the first study to identify endogenous and viral MHC class I ligands for any bat species and, as such, provides an important avenue for monitoring and development of vaccines against major bat-borne viruses both in the reservoir and spillover hosts. Additionally, it will provide a foundation to understand the role of adaptive immunity in bat antiviral responses.

UR - http://www.ncbi.nlm.nih.gov/pubmed/27183594

U2 - 10.4049/jimmunol.1502062

DO - 10.4049/jimmunol.1502062

M3 - Article

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EP - 4476

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -