Characterization of the α-amylase inhibitory activity of oligomeric proanthocyanidins from Acacia mearnsii bark extract

Yosuke Matsuo; Rie Kusano; Sosuke Ogawa; Yoshikazu Yazaki; Takashi Tanaka

doi:10.1177/1934578X1601101219

Characterization of the α-amylase inhibitory activity of oligomeric proanthocyanidins from Acacia mearnsii bark extract

Yosuke Matsuo, Rie Kusano, Sosuke Ogawa, Yoshikazu Yazaki, Takashi Tanaka

Chemical & Biological Engineering

Research output: Contribution to journal › Article › Other › peer-review

Abstract

Acacia mearnsii (Fabaceae) contains acacia polyphenols, which are a complex mixture of proanthocyanidins that are mainly composed of 5-deoxycatechin units. In this study, an aqueous extract of A. mearnsii bark was fractionated and the α-amylase inhibitory activity of each fraction was evaluated. The ¹³C NMR and MS data and the pyrolysis products obtained from the active and inactive fractions were compared. The spectroscopic results clearly indicated that fractions with strong inhibitory activity contained proanthocyanidin oligomers with catechol-type B-rings rather than pyrogallol-type B-rings. HPLC analysis of the pyrolysis products showed peaks for pyrocatechol were only observed in the mixtures obtained from the fractions with high inhibitory activities. In addition, (+)-pinitol was isolated as a major polyol of the extract at a level comparable with that of sucrose.

Original language	English
Pages (from-to)	1851-1854
Number of pages	4
Journal	Natural Product Communications
Volume	11
Issue number	12
DOIs	https://doi.org/10.1177/1934578X1601101219
Publication status	Published - 2016

Keywords

Acacia mearnsii
a-amylase inhibition
Proanthocyanidin
5-deoxycatechin
Pinitol

Access to Document

10.1177/1934578X1601101219

Cite this

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title = "Characterization of the α-amylase inhibitory activity of oligomeric proanthocyanidins from Acacia mearnsii bark extract",

abstract = "Acacia mearnsii (Fabaceae) contains acacia polyphenols, which are a complex mixture of proanthocyanidins that are mainly composed of 5-deoxycatechin units. In this study, an aqueous extract of A. mearnsii bark was fractionated and the α-amylase inhibitory activity of each fraction was evaluated. The 13C NMR and MS data and the pyrolysis products obtained from the active and inactive fractions were compared. The spectroscopic results clearly indicated that fractions with strong inhibitory activity contained proanthocyanidin oligomers with catechol-type B-rings rather than pyrogallol-type B-rings. HPLC analysis of the pyrolysis products showed peaks for pyrocatechol were only observed in the mixtures obtained from the fractions with high inhibitory activities. In addition, (+)-pinitol was isolated as a major polyol of the extract at a level comparable with that of sucrose.",

keywords = "Acacia mearnsii , a-amylase inhibition, Proanthocyanidin, 5-deoxycatechin, Pinitol",

author = "Yosuke Matsuo and Rie Kusano and Sosuke Ogawa and Yoshikazu Yazaki and Takashi Tanaka",

year = "2016",

doi = "10.1177/1934578X1601101219",

language = "English",

volume = "11",

pages = "1851--1854",

journal = "Natural Product Communications",

issn = "1934-578X",

publisher = "Natural Product Incorporation",

number = "12",

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TY - JOUR

T1 - Characterization of the α-amylase inhibitory activity of oligomeric proanthocyanidins from Acacia mearnsii bark extract

AU - Matsuo, Yosuke

AU - Kusano, Rie

AU - Ogawa, Sosuke

AU - Yazaki, Yoshikazu

AU - Tanaka, Takashi

PY - 2016

Y1 - 2016

N2 - Acacia mearnsii (Fabaceae) contains acacia polyphenols, which are a complex mixture of proanthocyanidins that are mainly composed of 5-deoxycatechin units. In this study, an aqueous extract of A. mearnsii bark was fractionated and the α-amylase inhibitory activity of each fraction was evaluated. The 13C NMR and MS data and the pyrolysis products obtained from the active and inactive fractions were compared. The spectroscopic results clearly indicated that fractions with strong inhibitory activity contained proanthocyanidin oligomers with catechol-type B-rings rather than pyrogallol-type B-rings. HPLC analysis of the pyrolysis products showed peaks for pyrocatechol were only observed in the mixtures obtained from the fractions with high inhibitory activities. In addition, (+)-pinitol was isolated as a major polyol of the extract at a level comparable with that of sucrose.

AB - Acacia mearnsii (Fabaceae) contains acacia polyphenols, which are a complex mixture of proanthocyanidins that are mainly composed of 5-deoxycatechin units. In this study, an aqueous extract of A. mearnsii bark was fractionated and the α-amylase inhibitory activity of each fraction was evaluated. The 13C NMR and MS data and the pyrolysis products obtained from the active and inactive fractions were compared. The spectroscopic results clearly indicated that fractions with strong inhibitory activity contained proanthocyanidin oligomers with catechol-type B-rings rather than pyrogallol-type B-rings. HPLC analysis of the pyrolysis products showed peaks for pyrocatechol were only observed in the mixtures obtained from the fractions with high inhibitory activities. In addition, (+)-pinitol was isolated as a major polyol of the extract at a level comparable with that of sucrose.

KW - Acacia mearnsii

KW - a-amylase inhibition

KW - Proanthocyanidin

KW - 5-deoxycatechin

KW - Pinitol

U2 - 10.1177/1934578X1601101219

DO - 10.1177/1934578X1601101219

M3 - Article

SN - 1934-578X

VL - 11

SP - 1851

EP - 1854

JO - Natural Product Communications

JF - Natural Product Communications

IS - 12

ER -