Abstract
Na-K-ATPase is a heterodimer of α- and β-subunits often referred to as the 'sodium pump' responsible for the maintenance of cell volume and electric potential across cell membranes. In the present study we have used antisense RNA probes to localize these subunits in the rat kidney by in situ hybridization. α1-Subunit and β-subunit gene expression are highest in cortical distal convoluted tubules, and the thick ascending limb of the loops of Henle; high in some proximal convoluted tubules, and low in the collecting ducts and blood vessels. Expression of the α11 and α111 isoforms is very low or absent thoughout the kidney. These results confirm and extend studies of Na-K-ATPase enzyme activity levels and immunocytochemical localization in the kidney.
Original language | English |
---|---|
Pages (from-to) | 10-15 |
Number of pages | 6 |
Journal | Renal Physiology and Biochemistry |
Volume | 15 |
Issue number | 1 |
Publication status | Published - 1992 |
Externally published | Yes |
Keywords
- Gene expression
- In situ hybridization
- Kidney
- Na-K-ATPase