Characterization of renal Na-K-ATPase gene expression by in situ hybridization

P. G P Charles, J. P. Dowling, P. J. Fuller

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Na-K-ATPase is a heterodimer of α- and β-subunits often referred to as the 'sodium pump' responsible for the maintenance of cell volume and electric potential across cell membranes. In the present study we have used antisense RNA probes to localize these subunits in the rat kidney by in situ hybridization. α1-Subunit and β-subunit gene expression are highest in cortical distal convoluted tubules, and the thick ascending limb of the loops of Henle; high in some proximal convoluted tubules, and low in the collecting ducts and blood vessels. Expression of the α11 and α111 isoforms is very low or absent thoughout the kidney. These results confirm and extend studies of Na-K-ATPase enzyme activity levels and immunocytochemical localization in the kidney.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalRenal Physiology and Biochemistry
Volume15
Issue number1
Publication statusPublished - 1992
Externally publishedYes

Keywords

  • Gene expression
  • In situ hybridization
  • Kidney
  • Na-K-ATPase

Cite this