Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells

Cameron N. Johnstone, Niall C. Tebbutt, Helen E. Abud, Sara J. White, Kaye L. Stenvers, Nathan E. Hall, Robert H. Whitehead, Stephen H. Cody, Bruno Catimel, Edouard C. Nice, Antony W. Burgess, Joan K. Heath

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

The murine A33 antigen is emerging as a definitive marker of intestinal epithelial cells. Cloning and sequence determination of cDNAs encoding mA33 antigen predict a novel type 1 transmembrane protein of 298 amino acids, comprising an extracellular domain with two immunoglobulin-like domains, a single-span transmembrane domain, and a highly acidic cytoplasmic domain. On the basis of conservation of amino acid sequence and genomic structure, the mA33 antigen is a member of a growing subfamily within the immunoglobulin superfamily, which includes transmembrane proteins CTX/ChT1, CTM/CTH, and CAR. During embryonic development, mA33 antigen expression is first observed in the inner cell mass of blastocysts before implantation. Intestinal expression of mA33 antigen is initiated in the hindgut at E14.5 and increases steadily throughout late embryonic and postnatal life into adulthood. The protein is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages, independent of their position along the rostrocaudal and crypt-villus axes. Thus the mA33 antigen appears to be a novel marker for both proliferating and differentiating intestinal epithelial cells.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume279
Issue number3 42-3
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Immunoglobulin superfamily
  • Intestinal epithelium

Cite this

Johnstone, Cameron N. ; Tebbutt, Niall C. ; Abud, Helen E. ; White, Sara J. ; Stenvers, Kaye L. ; Hall, Nathan E. ; Whitehead, Robert H. ; Cody, Stephen H. ; Catimel, Bruno ; Nice, Edouard C. ; Burgess, Antony W. ; Heath, Joan K. / Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2000 ; Vol. 279, No. 3 42-3.
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title = "Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells",
abstract = "The murine A33 antigen is emerging as a definitive marker of intestinal epithelial cells. Cloning and sequence determination of cDNAs encoding mA33 antigen predict a novel type 1 transmembrane protein of 298 amino acids, comprising an extracellular domain with two immunoglobulin-like domains, a single-span transmembrane domain, and a highly acidic cytoplasmic domain. On the basis of conservation of amino acid sequence and genomic structure, the mA33 antigen is a member of a growing subfamily within the immunoglobulin superfamily, which includes transmembrane proteins CTX/ChT1, CTM/CTH, and CAR. During embryonic development, mA33 antigen expression is first observed in the inner cell mass of blastocysts before implantation. Intestinal expression of mA33 antigen is initiated in the hindgut at E14.5 and increases steadily throughout late embryonic and postnatal life into adulthood. The protein is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages, independent of their position along the rostrocaudal and crypt-villus axes. Thus the mA33 antigen appears to be a novel marker for both proliferating and differentiating intestinal epithelial cells.",
keywords = "Immunoglobulin superfamily, Intestinal epithelium",
author = "Johnstone, {Cameron N.} and Tebbutt, {Niall C.} and Abud, {Helen E.} and White, {Sara J.} and Stenvers, {Kaye L.} and Hall, {Nathan E.} and Whitehead, {Robert H.} and Cody, {Stephen H.} and Bruno Catimel and Nice, {Edouard C.} and Burgess, {Antony W.} and Heath, {Joan K.}",
year = "2000",
language = "English",
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journal = "American Journal of Physiology - Gastrointestinal and Liver Physiology",
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Johnstone, CN, Tebbutt, NC, Abud, HE, White, SJ, Stenvers, KL, Hall, NE, Whitehead, RH, Cody, SH, Catimel, B, Nice, EC, Burgess, AW & Heath, JK 2000, 'Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 279, no. 3 42-3.

Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells. / Johnstone, Cameron N.; Tebbutt, Niall C.; Abud, Helen E.; White, Sara J.; Stenvers, Kaye L.; Hall, Nathan E.; Whitehead, Robert H.; Cody, Stephen H.; Catimel, Bruno; Nice, Edouard C.; Burgess, Antony W.; Heath, Joan K.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 279, No. 3 42-3, 2000.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells

AU - Johnstone, Cameron N.

AU - Tebbutt, Niall C.

AU - Abud, Helen E.

AU - White, Sara J.

AU - Stenvers, Kaye L.

AU - Hall, Nathan E.

AU - Whitehead, Robert H.

AU - Cody, Stephen H.

AU - Catimel, Bruno

AU - Nice, Edouard C.

AU - Burgess, Antony W.

AU - Heath, Joan K.

PY - 2000

Y1 - 2000

N2 - The murine A33 antigen is emerging as a definitive marker of intestinal epithelial cells. Cloning and sequence determination of cDNAs encoding mA33 antigen predict a novel type 1 transmembrane protein of 298 amino acids, comprising an extracellular domain with two immunoglobulin-like domains, a single-span transmembrane domain, and a highly acidic cytoplasmic domain. On the basis of conservation of amino acid sequence and genomic structure, the mA33 antigen is a member of a growing subfamily within the immunoglobulin superfamily, which includes transmembrane proteins CTX/ChT1, CTM/CTH, and CAR. During embryonic development, mA33 antigen expression is first observed in the inner cell mass of blastocysts before implantation. Intestinal expression of mA33 antigen is initiated in the hindgut at E14.5 and increases steadily throughout late embryonic and postnatal life into adulthood. The protein is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages, independent of their position along the rostrocaudal and crypt-villus axes. Thus the mA33 antigen appears to be a novel marker for both proliferating and differentiating intestinal epithelial cells.

AB - The murine A33 antigen is emerging as a definitive marker of intestinal epithelial cells. Cloning and sequence determination of cDNAs encoding mA33 antigen predict a novel type 1 transmembrane protein of 298 amino acids, comprising an extracellular domain with two immunoglobulin-like domains, a single-span transmembrane domain, and a highly acidic cytoplasmic domain. On the basis of conservation of amino acid sequence and genomic structure, the mA33 antigen is a member of a growing subfamily within the immunoglobulin superfamily, which includes transmembrane proteins CTX/ChT1, CTM/CTH, and CAR. During embryonic development, mA33 antigen expression is first observed in the inner cell mass of blastocysts before implantation. Intestinal expression of mA33 antigen is initiated in the hindgut at E14.5 and increases steadily throughout late embryonic and postnatal life into adulthood. The protein is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages, independent of their position along the rostrocaudal and crypt-villus axes. Thus the mA33 antigen appears to be a novel marker for both proliferating and differentiating intestinal epithelial cells.

KW - Immunoglobulin superfamily

KW - Intestinal epithelium

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M3 - Article

VL - 279

JO - American Journal of Physiology - Gastrointestinal and Liver Physiology

JF - American Journal of Physiology - Gastrointestinal and Liver Physiology

SN - 0193-1857

IS - 3 42-3

ER -