Characterization of Monofunctional Chorismate Mutase/Prephenate Dehydrogenase Enzymes Obtained via Mutagenesis of Recombinant Plasmids in vitro

Julian I. ROOD, Brigitte PERROT, Elizabeth HEYDE, John F. MORRISON

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Mutagenesis in vitro has been used to obtain mutant forms of the bifunctional enzyme, chorismate mutase/ prephenate dehydrogenase. Plasmid DNA containing the genes that code for the enzyme was treated with hydroxylamine and the resulting products were used to transform strains of Eschericliia coli. Two types of mutant were isolated. One contained enzyme which was niutase‐positive, dehydrogenase‐negative while the other not exhibit either activity. Kinetic and physical analysis of one of the purified monofunctional enzymes showed that the loss of dehydrogenase activity was due to modification of the binding site for NAD. The results open the way for molecular studies of structure‐function relationships with this bifunctional enzyme.

Original languageEnglish
Pages (from-to)513-519
Number of pages7
JournalEuropean Journal of Biochemistry
Volume124
Issue number3
DOIs
Publication statusPublished - 1 Jan 1982
Externally publishedYes

Cite this

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abstract = "Mutagenesis in vitro has been used to obtain mutant forms of the bifunctional enzyme, chorismate mutase/ prephenate dehydrogenase. Plasmid DNA containing the genes that code for the enzyme was treated with hydroxylamine and the resulting products were used to transform strains of Eschericliia coli. Two types of mutant were isolated. One contained enzyme which was niutase‐positive, dehydrogenase‐negative while the other not exhibit either activity. Kinetic and physical analysis of one of the purified monofunctional enzymes showed that the loss of dehydrogenase activity was due to modification of the binding site for NAD. The results open the way for molecular studies of structure‐function relationships with this bifunctional enzyme.",
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Characterization of Monofunctional Chorismate Mutase/Prephenate Dehydrogenase Enzymes Obtained via Mutagenesis of Recombinant Plasmids in vitro. / ROOD, Julian I.; PERROT, Brigitte; HEYDE, Elizabeth; MORRISON, John F.

In: European Journal of Biochemistry, Vol. 124, No. 3, 01.01.1982, p. 513-519.

Research output: Contribution to journalArticleResearchpeer-review

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AU - ROOD, Julian I.

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