Characterization of Human Mucosal-associated Invariant T (MAIT) Cells

Michael N.T. Souter; Liyen Loh; Shihan Li; Bronwyn S. Meehan; Nicholas A. Gherardin; Dale I. Godfrey; Jamie Rossjohn; David P. Fairlie; Katherine Kedzierska; Daniel G. Pellicci; Zhenjun Chen; Lars Kjer-Nielsen; Alexandra J. Corbett; James McCluskey; Sidonia B.G. Eckle

doi:10.1002/cpim.90

Characterization of Human Mucosal-associated Invariant T (MAIT) Cells

Michael N.T. Souter, Liyen Loh, Shihan Li, Bronwyn S. Meehan, Nicholas A. Gherardin, Dale I. Godfrey, Jamie Rossjohn, David P. Fairlie, Katherine Kedzierska, Daniel G. Pellicci, Zhenjun Chen, Lars Kjer-Nielsen, Alexandra J. Corbett, James McCluskey, Sidonia B.G. Eckle

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Abstract

Mucosal-associated invariant T (MAIT) cells are a subset of unconventional T cells restricted by the major histocompatibility complex (MHC) class I–like molecule MHC-related protein 1 (MR1). MAIT cells are found throughout the body, especially in human blood and liver. Unlike conventional T cells, which are stimulated by peptide antigens presented by MHC molecules, MAIT cells recognize metabolite antigens derived from an intermediate in the microbial biosynthesis of riboflavin. MAIT cells mediate protective immunity to infections by riboflavin-producing microbes via the production of cytokines and cytotoxicity. The discovery of stimulating MAIT cell antigens allowed for the development of an analytical tool, the MR1 tetramer, that binds specifically to the MAIT T cell receptor (TCR) and is becoming the gold standard for identification of MAIT cells by flow cytometry. This article describes protocols to characterize the phenotype of human MAIT cells in blood and tissues by flow cytometry using fluorescently labeled human MR1 tetramers alongside antibodies specific for MAIT cell markers.

Original language	English
Article number	e90
Number of pages	61
Journal	Current Protocols in Immunology
Volume	127
Issue number	1
DOIs	https://doi.org/10.1002/cpim.90
Publication status	Published - Dec 2019

Keywords

antigen
flow cytometry
human
immune system
MAIT cells
metabolite
MR1
riboflavin
tetramer

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10.1002/cpim.90

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Souter, M. N. T., Loh, L., Li, S., Meehan, B. S., Gherardin, N. A., Godfrey, D. I., Rossjohn, J., Fairlie, D. P., Kedzierska, K., Pellicci, D. G., Chen, Z., Kjer-Nielsen, L., Corbett, A. J., McCluskey, J., & Eckle, S. B. G. (2019). Characterization of Human Mucosal-associated Invariant T (MAIT) Cells. Current Protocols in Immunology, 127(1), [e90]. https://doi.org/10.1002/cpim.90

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abstract = "Mucosal-associated invariant T (MAIT) cells are a subset of unconventional T cells restricted by the major histocompatibility complex (MHC) class I–like molecule MHC-related protein 1 (MR1). MAIT cells are found throughout the body, especially in human blood and liver. Unlike conventional T cells, which are stimulated by peptide antigens presented by MHC molecules, MAIT cells recognize metabolite antigens derived from an intermediate in the microbial biosynthesis of riboflavin. MAIT cells mediate protective immunity to infections by riboflavin-producing microbes via the production of cytokines and cytotoxicity. The discovery of stimulating MAIT cell antigens allowed for the development of an analytical tool, the MR1 tetramer, that binds specifically to the MAIT T cell receptor (TCR) and is becoming the gold standard for identification of MAIT cells by flow cytometry. This article describes protocols to characterize the phenotype of human MAIT cells in blood and tissues by flow cytometry using fluorescently labeled human MR1 tetramers alongside antibodies specific for MAIT cell markers.",

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Characterization of Human Mucosal-associated Invariant T (MAIT) Cells

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Keywords

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ARC Centre of Excellence in Advanced Molecular Imaging

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Characterization of Human Mucosal-associated Invariant T (MAIT) Cells

Abstract

Keywords

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ARC Centre of Excellence in Advanced Molecular Imaging

Cite this