Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W Andrews, Gemma Beighton, Paul Bello, Nissim Benvenisty, Lorraine S Berry, Simon Bevan, Barak Blum, Justin Brooking, Kevin G Chen, Andre BH Choo, Gary A Churchill, Marie Corbel, Ivan Damjanov, John S Draper, Petr Dvorak, Katarina Emanuelsson & 65 others Roland A Fleck, Angela Ford, Karin Astrid Maria Gertow, Marina Gertsenstein, Paul J Gokhale, Rebecca S Hamilton, Alex Hampl, Lyn E Healy, Outi Hovatta, Johan Hyllner, Marta P Imreh, Joseph Itskovitz-Eldor, Jamie Jackson, Jackie Johnson, Mark Jones, Kehkooi Kee, Benjamin L King, Barbara B Knowles, Majlinda Lako, Franck Lebrin, Barbara S Mallon, Daisy Manning, Yoav Mayshar, Ron McKay, Anna Emilia Michalska, Milla Mikkola, Masha Mileikovsky, Stephen L Minger, Harry D Moore, Christine L Mummery, Andras Nagy, Norio Nakatsuji, Carmel O'Brien, Steve K Oh, Cia Olsson, Timo Otonkoski, Kye-Yoon Park, Robert Passier, Hema Patel, Minal Patel, Roger Pedersen, Martin Frederick Pera, Marian S Piekarczyk, Renee A Reijo Pera, Benjamin E Reubinoff, Allan J Robins, Janet Rossant, Peter Rugg-Gunn, Thomas C Schulz, Henrik Semb, Eric S Sherrer, Henrike Siemen, Glyn N Stacey, Miodrag Stojkovic, Hirofumi Suemori, Jin Szatkiewicz, Tikva Turetsky, Timo Tuuri, Steineke van den Brink, Kristina Vintersten, Sanna Vuoristo, Dorien Ward, Thomas A Weaver, Lesley A Young, Weidong Zhang

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
Original languageEnglish
Pages (from-to)803 - 816
Number of pages14
JournalNature Biotechnology
Volume25
Issue number7
Publication statusPublished - 2007

Cite this

Adewumi, O., Aflatoonian, B., Ahrlund-Richter, L., Amit, M., Andrews, P. W., Beighton, G., ... Zhang, W. (2007). Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature Biotechnology, 25(7), 803 - 816.
Adewumi, Oluseun ; Aflatoonian, Behrouz ; Ahrlund-Richter, Lars ; Amit, Michal ; Andrews, Peter W ; Beighton, Gemma ; Bello, Paul ; Benvenisty, Nissim ; Berry, Lorraine S ; Bevan, Simon ; Blum, Barak ; Brooking, Justin ; Chen, Kevin G ; Choo, Andre BH ; Churchill, Gary A ; Corbel, Marie ; Damjanov, Ivan ; Draper, John S ; Dvorak, Petr ; Emanuelsson, Katarina ; Fleck, Roland A ; Ford, Angela ; Gertow, Karin Astrid Maria ; Gertsenstein, Marina ; Gokhale, Paul J ; Hamilton, Rebecca S ; Hampl, Alex ; Healy, Lyn E ; Hovatta, Outi ; Hyllner, Johan ; Imreh, Marta P ; Itskovitz-Eldor, Joseph ; Jackson, Jamie ; Johnson, Jackie ; Jones, Mark ; Kee, Kehkooi ; King, Benjamin L ; Knowles, Barbara B ; Lako, Majlinda ; Lebrin, Franck ; Mallon, Barbara S ; Manning, Daisy ; Mayshar, Yoav ; McKay, Ron ; Michalska, Anna Emilia ; Mikkola, Milla ; Mileikovsky, Masha ; Minger, Stephen L ; Moore, Harry D ; Mummery, Christine L ; Nagy, Andras ; Nakatsuji, Norio ; O'Brien, Carmel ; Oh, Steve K ; Olsson, Cia ; Otonkoski, Timo ; Park, Kye-Yoon ; Passier, Robert ; Patel, Hema ; Patel, Minal ; Pedersen, Roger ; Pera, Martin Frederick ; Piekarczyk, Marian S ; Reijo Pera, Renee A ; Reubinoff, Benjamin E ; Robins, Allan J ; Rossant, Janet ; Rugg-Gunn, Peter ; Schulz, Thomas C ; Semb, Henrik ; Sherrer, Eric S ; Siemen, Henrike ; Stacey, Glyn N ; Stojkovic, Miodrag ; Suemori, Hirofumi ; Szatkiewicz, Jin ; Turetsky, Tikva ; Tuuri, Timo ; van den Brink, Steineke ; Vintersten, Kristina ; Vuoristo, Sanna ; Ward, Dorien ; Weaver, Thomas A ; Young, Lesley A ; Zhang, Weidong. / Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. In: Nature Biotechnology. 2007 ; Vol. 25, No. 7. pp. 803 - 816.
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abstract = "The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.",
author = "Oluseun Adewumi and Behrouz Aflatoonian and Lars Ahrlund-Richter and Michal Amit and Andrews, {Peter W} and Gemma Beighton and Paul Bello and Nissim Benvenisty and Berry, {Lorraine S} and Simon Bevan and Barak Blum and Justin Brooking and Chen, {Kevin G} and Choo, {Andre BH} and Churchill, {Gary A} and Marie Corbel and Ivan Damjanov and Draper, {John S} and Petr Dvorak and Katarina Emanuelsson and Fleck, {Roland A} and Angela Ford and Gertow, {Karin Astrid Maria} and Marina Gertsenstein and Gokhale, {Paul J} and Hamilton, {Rebecca S} and Alex Hampl and Healy, {Lyn E} and Outi Hovatta and Johan Hyllner and Imreh, {Marta P} and Joseph Itskovitz-Eldor and Jamie Jackson and Jackie Johnson and Mark Jones and Kehkooi Kee and King, {Benjamin L} and Knowles, {Barbara B} and Majlinda Lako and Franck Lebrin and Mallon, {Barbara S} and Daisy Manning and Yoav Mayshar and Ron McKay and Michalska, {Anna Emilia} and Milla Mikkola and Masha Mileikovsky and Minger, {Stephen L} and Moore, {Harry D} and Mummery, {Christine L} and Andras Nagy and Norio Nakatsuji and Carmel O'Brien and Oh, {Steve K} and Cia Olsson and Timo Otonkoski and Kye-Yoon Park and Robert Passier and Hema Patel and Minal Patel and Roger Pedersen and Pera, {Martin Frederick} and Piekarczyk, {Marian S} and {Reijo Pera}, {Renee A} and Reubinoff, {Benjamin E} and Robins, {Allan J} and Janet Rossant and Peter Rugg-Gunn and Schulz, {Thomas C} and Henrik Semb and Sherrer, {Eric S} and Henrike Siemen and Stacey, {Glyn N} and Miodrag Stojkovic and Hirofumi Suemori and Jin Szatkiewicz and Tikva Turetsky and Timo Tuuri and {van den Brink}, Steineke and Kristina Vintersten and Sanna Vuoristo and Dorien Ward and Weaver, {Thomas A} and Young, {Lesley A} and Weidong Zhang",
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Adewumi, O, Aflatoonian, B, Ahrlund-Richter, L, Amit, M, Andrews, PW, Beighton, G, Bello, P, Benvenisty, N, Berry, LS, Bevan, S, Blum, B, Brooking, J, Chen, KG, Choo, ABH, Churchill, GA, Corbel, M, Damjanov, I, Draper, JS, Dvorak, P, Emanuelsson, K, Fleck, RA, Ford, A, Gertow, KAM, Gertsenstein, M, Gokhale, PJ, Hamilton, RS, Hampl, A, Healy, LE, Hovatta, O, Hyllner, J, Imreh, MP, Itskovitz-Eldor, J, Jackson, J, Johnson, J, Jones, M, Kee, K, King, BL, Knowles, BB, Lako, M, Lebrin, F, Mallon, BS, Manning, D, Mayshar, Y, McKay, R, Michalska, AE, Mikkola, M, Mileikovsky, M, Minger, SL, Moore, HD, Mummery, CL, Nagy, A, Nakatsuji, N, O'Brien, C, Oh, SK, Olsson, C, Otonkoski, T, Park, K-Y, Passier, R, Patel, H, Patel, M, Pedersen, R, Pera, MF, Piekarczyk, MS, Reijo Pera, RA, Reubinoff, BE, Robins, AJ, Rossant, J, Rugg-Gunn, P, Schulz, TC, Semb, H, Sherrer, ES, Siemen, H, Stacey, GN, Stojkovic, M, Suemori, H, Szatkiewicz, J, Turetsky, T, Tuuri, T, van den Brink, S, Vintersten, K, Vuoristo, S, Ward, D, Weaver, TA, Young, LA & Zhang, W 2007, 'Characterization of human embryonic stem cell lines by the International Stem Cell Initiative' Nature Biotechnology, vol. 25, no. 7, pp. 803 - 816.

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. / Adewumi, Oluseun; Aflatoonian, Behrouz; Ahrlund-Richter, Lars; Amit, Michal; Andrews, Peter W; Beighton, Gemma; Bello, Paul; Benvenisty, Nissim; Berry, Lorraine S; Bevan, Simon; Blum, Barak; Brooking, Justin; Chen, Kevin G; Choo, Andre BH; Churchill, Gary A; Corbel, Marie; Damjanov, Ivan; Draper, John S; Dvorak, Petr; Emanuelsson, Katarina; Fleck, Roland A; Ford, Angela; Gertow, Karin Astrid Maria; Gertsenstein, Marina; Gokhale, Paul J; Hamilton, Rebecca S; Hampl, Alex; Healy, Lyn E; Hovatta, Outi; Hyllner, Johan; Imreh, Marta P; Itskovitz-Eldor, Joseph; Jackson, Jamie; Johnson, Jackie; Jones, Mark; Kee, Kehkooi; King, Benjamin L; Knowles, Barbara B; Lako, Majlinda; Lebrin, Franck; Mallon, Barbara S; Manning, Daisy; Mayshar, Yoav; McKay, Ron; Michalska, Anna Emilia; Mikkola, Milla; Mileikovsky, Masha; Minger, Stephen L; Moore, Harry D; Mummery, Christine L; Nagy, Andras; Nakatsuji, Norio; O'Brien, Carmel; Oh, Steve K; Olsson, Cia; Otonkoski, Timo; Park, Kye-Yoon; Passier, Robert; Patel, Hema; Patel, Minal; Pedersen, Roger; Pera, Martin Frederick; Piekarczyk, Marian S; Reijo Pera, Renee A; Reubinoff, Benjamin E; Robins, Allan J; Rossant, Janet; Rugg-Gunn, Peter; Schulz, Thomas C; Semb, Henrik; Sherrer, Eric S; Siemen, Henrike; Stacey, Glyn N; Stojkovic, Miodrag; Suemori, Hirofumi; Szatkiewicz, Jin; Turetsky, Tikva; Tuuri, Timo; van den Brink, Steineke; Vintersten, Kristina; Vuoristo, Sanna; Ward, Dorien; Weaver, Thomas A; Young, Lesley A; Zhang, Weidong.

In: Nature Biotechnology, Vol. 25, No. 7, 2007, p. 803 - 816.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

AU - Adewumi, Oluseun

AU - Aflatoonian, Behrouz

AU - Ahrlund-Richter, Lars

AU - Amit, Michal

AU - Andrews, Peter W

AU - Beighton, Gemma

AU - Bello, Paul

AU - Benvenisty, Nissim

AU - Berry, Lorraine S

AU - Bevan, Simon

AU - Blum, Barak

AU - Brooking, Justin

AU - Chen, Kevin G

AU - Choo, Andre BH

AU - Churchill, Gary A

AU - Corbel, Marie

AU - Damjanov, Ivan

AU - Draper, John S

AU - Dvorak, Petr

AU - Emanuelsson, Katarina

AU - Fleck, Roland A

AU - Ford, Angela

AU - Gertow, Karin Astrid Maria

AU - Gertsenstein, Marina

AU - Gokhale, Paul J

AU - Hamilton, Rebecca S

AU - Hampl, Alex

AU - Healy, Lyn E

AU - Hovatta, Outi

AU - Hyllner, Johan

AU - Imreh, Marta P

AU - Itskovitz-Eldor, Joseph

AU - Jackson, Jamie

AU - Johnson, Jackie

AU - Jones, Mark

AU - Kee, Kehkooi

AU - King, Benjamin L

AU - Knowles, Barbara B

AU - Lako, Majlinda

AU - Lebrin, Franck

AU - Mallon, Barbara S

AU - Manning, Daisy

AU - Mayshar, Yoav

AU - McKay, Ron

AU - Michalska, Anna Emilia

AU - Mikkola, Milla

AU - Mileikovsky, Masha

AU - Minger, Stephen L

AU - Moore, Harry D

AU - Mummery, Christine L

AU - Nagy, Andras

AU - Nakatsuji, Norio

AU - O'Brien, Carmel

AU - Oh, Steve K

AU - Olsson, Cia

AU - Otonkoski, Timo

AU - Park, Kye-Yoon

AU - Passier, Robert

AU - Patel, Hema

AU - Patel, Minal

AU - Pedersen, Roger

AU - Pera, Martin Frederick

AU - Piekarczyk, Marian S

AU - Reijo Pera, Renee A

AU - Reubinoff, Benjamin E

AU - Robins, Allan J

AU - Rossant, Janet

AU - Rugg-Gunn, Peter

AU - Schulz, Thomas C

AU - Semb, Henrik

AU - Sherrer, Eric S

AU - Siemen, Henrike

AU - Stacey, Glyn N

AU - Stojkovic, Miodrag

AU - Suemori, Hirofumi

AU - Szatkiewicz, Jin

AU - Turetsky, Tikva

AU - Tuuri, Timo

AU - van den Brink, Steineke

AU - Vintersten, Kristina

AU - Vuoristo, Sanna

AU - Ward, Dorien

AU - Weaver, Thomas A

AU - Young, Lesley A

AU - Zhang, Weidong

PY - 2007

Y1 - 2007

N2 - The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

AB - The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17572666

M3 - Article

VL - 25

SP - 803

EP - 816

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 7

ER -

Adewumi O, Aflatoonian B, Ahrlund-Richter L, Amit M, Andrews PW, Beighton G et al. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature Biotechnology. 2007;25(7):803 - 816.