Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W Andrews, Gemma Beighton, Paul Bello, Nissim Benvenisty, Lorraine S Berry, Simon Bevan, Barak Blum, Justin Brooking, Kevin G Chen, Andre BH Choo, Gary A Churchill, Marie Corbel, Ivan Damjanov, John S Draper, Petr Dvorak, Katarina EmanuelssonRoland A Fleck, Angela Ford, Karin Astrid Maria Gertow, Marina Gertsenstein, Paul J Gokhale, Rebecca S Hamilton, Alex Hampl, Lyn E Healy, Outi Hovatta, Johan Hyllner, Marta P Imreh, Joseph Itskovitz-Eldor, Jamie Jackson, Jackie Johnson, Mark Jones, Kehkooi Kee, Benjamin L King, Barbara B Knowles, Majlinda Lako, Franck Lebrin, Barbara S Mallon, Daisy Manning, Yoav Mayshar, Ron McKay, Anna Emilia Michalska, Milla Mikkola, Masha Mileikovsky, Stephen L Minger, Harry D Moore, Christine L Mummery, Andras Nagy, Norio Nakatsuji, Carmel O'Brien, Steve K Oh, Cia Olsson, Timo Otonkoski, Kye-Yoon Park, Robert Passier, Hema Patel, Minal Patel, Roger Pedersen, Martin Frederick Pera, Marian S Piekarczyk, Renee A Reijo Pera, Benjamin E Reubinoff, Allan J Robins, Janet Rossant, Peter Rugg-Gunn, Thomas C Schulz, Henrik Semb, Eric S Sherrer, Henrike Siemen, Glyn N Stacey, Miodrag Stojkovic, Hirofumi Suemori, Jin Szatkiewicz, Tikva Turetsky, Timo Tuuri, Steineke van den Brink, Kristina Vintersten, Sanna Vuoristo, Dorien Ward, Thomas A Weaver, Lesley A Young, Weidong Zhang

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The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
Original languageEnglish
Pages (from-to)803 - 816
Number of pages14
JournalNature Biotechnology
Issue number7
Publication statusPublished - 2007

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