Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein: A Force-Pulling Study

Pascale R. Leroueil, Stassi DiMaggio, Abigail N. Leistra, Craig D. Blanchette, Christine Orme, Kumar Sinniah, Bradford G. Orr, Mark M. Banaszak Holl

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Atomic force microscopy force-pulling experiments have been used to measure the binding forces between folic acid (FA) conjugated poly(amidoamine) (PAMAM) dendrimers and folate binding protein (FBP). The generation 5 (G5) PAMAM conjugates contained an average of 2.7, 4.7, and 7.2 FA per dendrimer. The most probable rupture force was measured to be 83, 201, and 189 pN for G5-FA2.7, G5-FA4.7, and G5-FA7.2, respectively. Folic acid blocking experiments for G5-FA7.2 reduced the frequency of successful binding events and increased the magnitude of the average rupture force to 274 pN. The force data are interpreted as arising from a network of van der Waals and electrostatic interactions that form between FBP and G5 PAMAM dendrimer, resulting in a binding strength far greater than that expected for an interaction between FA and FBP alone. (Graph presented).

Original languageEnglish
Pages (from-to)11506-11512
Number of pages7
JournalJournal of Physical Chemistry B
Volume119
Issue number35
DOIs
Publication statusPublished - 3 Sep 2015
Externally publishedYes

Cite this

Leroueil, Pascale R. ; DiMaggio, Stassi ; Leistra, Abigail N. ; Blanchette, Craig D. ; Orme, Christine ; Sinniah, Kumar ; Orr, Bradford G. ; Banaszak Holl, Mark M. / Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein : A Force-Pulling Study. In: Journal of Physical Chemistry B. 2015 ; Vol. 119, No. 35. pp. 11506-11512.
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title = "Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein: A Force-Pulling Study",
abstract = "Atomic force microscopy force-pulling experiments have been used to measure the binding forces between folic acid (FA) conjugated poly(amidoamine) (PAMAM) dendrimers and folate binding protein (FBP). The generation 5 (G5) PAMAM conjugates contained an average of 2.7, 4.7, and 7.2 FA per dendrimer. The most probable rupture force was measured to be 83, 201, and 189 pN for G5-FA2.7, G5-FA4.7, and G5-FA7.2, respectively. Folic acid blocking experiments for G5-FA7.2 reduced the frequency of successful binding events and increased the magnitude of the average rupture force to 274 pN. The force data are interpreted as arising from a network of van der Waals and electrostatic interactions that form between FBP and G5 PAMAM dendrimer, resulting in a binding strength far greater than that expected for an interaction between FA and FBP alone. (Graph presented).",
author = "Leroueil, {Pascale R.} and Stassi DiMaggio and Leistra, {Abigail N.} and Blanchette, {Craig D.} and Christine Orme and Kumar Sinniah and Orr, {Bradford G.} and {Banaszak Holl}, {Mark M.}",
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Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein : A Force-Pulling Study. / Leroueil, Pascale R.; DiMaggio, Stassi; Leistra, Abigail N.; Blanchette, Craig D.; Orme, Christine; Sinniah, Kumar; Orr, Bradford G.; Banaszak Holl, Mark M.

In: Journal of Physical Chemistry B, Vol. 119, No. 35, 03.09.2015, p. 11506-11512.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Characterization of Folic Acid and Poly(amidoamine) Dendrimer Interactions with Folate Binding Protein

T2 - A Force-Pulling Study

AU - Leroueil, Pascale R.

AU - DiMaggio, Stassi

AU - Leistra, Abigail N.

AU - Blanchette, Craig D.

AU - Orme, Christine

AU - Sinniah, Kumar

AU - Orr, Bradford G.

AU - Banaszak Holl, Mark M.

PY - 2015/9/3

Y1 - 2015/9/3

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AB - Atomic force microscopy force-pulling experiments have been used to measure the binding forces between folic acid (FA) conjugated poly(amidoamine) (PAMAM) dendrimers and folate binding protein (FBP). The generation 5 (G5) PAMAM conjugates contained an average of 2.7, 4.7, and 7.2 FA per dendrimer. The most probable rupture force was measured to be 83, 201, and 189 pN for G5-FA2.7, G5-FA4.7, and G5-FA7.2, respectively. Folic acid blocking experiments for G5-FA7.2 reduced the frequency of successful binding events and increased the magnitude of the average rupture force to 274 pN. The force data are interpreted as arising from a network of van der Waals and electrostatic interactions that form between FBP and G5 PAMAM dendrimer, resulting in a binding strength far greater than that expected for an interaction between FA and FBP alone. (Graph presented).

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