Characterization of fibrinogen adsorption onto glass microcapillary surfaces by ELISA

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Adsorption of biomolecules onto microchannel surfaces remains a critical issue in microfluidic devices. This paper investigates the adsorption of fibrinogen on glass microcapillaries using an immunoassay method (ELISA) and X-ray photoelectron spectroscopy (XPS). Various adsorption conditions such as protein concentrations and incubation times, buffer pH, buffer ionic strengths and effects of flow are presented. ELISA is successfully demonstrated as a facile and robust technique to examine these phenomena. The highest adsorption level occurs near the isoelectric point of fibrinogen (pH 5.0) and low buffer ionic strengths (0–8 mM). Microchannel surface saturation was achieved at a fibrinogen solution concentration of ∼50 µg ml−1. Fibrinogen adsorption under flow was always higher than that seen in static systems. The importance of diffusion phenomena in microchannels on protein adsorption was demonstrated. ELISA experiments using fused silica and PEEK have also confirmed significant adsorption on these mass spectrometer transfer line materials.
Original languageEnglish
Pages (from-to)64-70
JournalLab on a Chip
Volume7
Publication statusPublished - 2007

Cite this