TY - JOUR
T1 - Characterization of choline chloride-based deep eutectic solvent for anthocyanin extraction via aqueous two-phase system
T2 - Physicochemical properties and liquid–liquid equilibrium phase diagram
AU - Meng, Xiaoqin
AU - Nalatambi, Sathishkumar
AU - Mahaindran, Adityanindran
AU - Tee, Lee Hong
AU - Chua, Bee Lin
AU - Oh, Kai Siang
AU - Lam, Weng Hoong
N1 - Funding Information:
The authors would like to acknowledge Ministry of Higher Education, Malaysia for providing financial support under the Fundamental Research Grant Scheme, FRGS/1/2020/TK0/TAYLOR/03/1.
Publisher Copyright:
© 2023 Curtin University and John Wiley & Sons Ltd.
PY - 2023/11
Y1 - 2023/11
N2 - The purpose of this research is to characterize and explore the feasibility of deep eutectic solvent-based aqueous two-phase system (DES-ATPS) for its potential application in the extraction and purification of anthocyanins. Various combinations and molar ratios of DES using choline chloride as hydrogen bond acceptor (HBA) and butanediol (BDO), ethylene glycol (EG), and lactic acid (LA) as hydrogen bond donor (HBD) were synthesized, screened, and investigated on its physicochemical properties. The binodal phase diagrams were developed as basis for the ATPS development. It was noted that LA-based natural deep eutectic solvents (NADES) were highly polar and likely to enhance the solubility of anthocyanin in DES. Regardless, the simple molecular structure and low viscosity of EG- and BDO-based DES enhanced the solubility of anthocyanin and recorded a monomeric anthocyanin content ranging from 10.52 ± 0.78 mg cyanidin-3-glucoside/g at 40°C and 10.78 ± 0.84 mg cyanidin-3-glucoside/g at 30°C, respectively. Lastly, the liquid–liquid equilibrium binodal curves were developed and ChCl:1,3-BDO (1:4) and ChCl:1,4-BDO (1:4) exhibited excellent phase-forming ability, thus concluding its excellent potential for anthocyanin extraction through ATPS.
AB - The purpose of this research is to characterize and explore the feasibility of deep eutectic solvent-based aqueous two-phase system (DES-ATPS) for its potential application in the extraction and purification of anthocyanins. Various combinations and molar ratios of DES using choline chloride as hydrogen bond acceptor (HBA) and butanediol (BDO), ethylene glycol (EG), and lactic acid (LA) as hydrogen bond donor (HBD) were synthesized, screened, and investigated on its physicochemical properties. The binodal phase diagrams were developed as basis for the ATPS development. It was noted that LA-based natural deep eutectic solvents (NADES) were highly polar and likely to enhance the solubility of anthocyanin in DES. Regardless, the simple molecular structure and low viscosity of EG- and BDO-based DES enhanced the solubility of anthocyanin and recorded a monomeric anthocyanin content ranging from 10.52 ± 0.78 mg cyanidin-3-glucoside/g at 40°C and 10.78 ± 0.84 mg cyanidin-3-glucoside/g at 30°C, respectively. Lastly, the liquid–liquid equilibrium binodal curves were developed and ChCl:1,3-BDO (1:4) and ChCl:1,4-BDO (1:4) exhibited excellent phase-forming ability, thus concluding its excellent potential for anthocyanin extraction through ATPS.
KW - anthocyanins
KW - aqueous two-phase system
KW - deep eutectic solvent
KW - extraction
KW - green solvent
KW - physicochemical characteristics
UR - http://www.scopus.com/inward/record.url?scp=85173530497&partnerID=8YFLogxK
U2 - 10.1002/apj.2990
DO - 10.1002/apj.2990
M3 - Article
AN - SCOPUS:85173530497
SN - 1932-2135
VL - 18
JO - Asia-Pacific Journal of Chemical Engineering
JF - Asia-Pacific Journal of Chemical Engineering
IS - 6
M1 - e2990
ER -
