Characterization of bovine heparin-binding neurotrophic factor (HBNF): Assignment of disulfide bonds

L. Fabri; E. C. Nice; L. D. Ward; H. Maruta; A. W. Burgess; R. J. Simpson

Characterization of bovine heparin-binding neurotrophic factor (HBNF): Assignment of disulfide bonds

L. Fabri, E. C. Nice, L. D. Ward, H. Maruta, A. W. Burgess, R. J. Simpson

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys₁₅-Cys₄₄, Cys₂₃-Cys₅₃, Cys₃₀-Cys₅₇, Cys₆₇-Cys₉₉ and Cys₇₇-Cys₁₀₉.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Biochemistry International
Volume	28
Issue number	1
Publication status	Published - 1 Jan 1992
Externally published	Yes

Cite this

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title = "Characterization of bovine heparin-binding neurotrophic factor (HBNF): Assignment of disulfide bonds",

abstract = "The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.",

author = "L. Fabri and Nice, \{E. C.\} and Ward, \{L. D.\} and H. Maruta and Burgess, \{A. W.\} and Simpson, \{R. J.\}",

year = "1992",

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language = "English",

volume = "28",

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journal = "Biochemistry International",

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TY - JOUR

T1 - Characterization of bovine heparin-binding neurotrophic factor (HBNF)

T2 - Assignment of disulfide bonds

AU - Fabri, L.

AU - Nice, E. C.

AU - Ward, L. D.

AU - Maruta, H.

AU - Burgess, A. W.

AU - Simpson, R. J.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.

AB - The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.

UR - https://www.scopus.com/pages/publications/0026669893

M3 - Article

C2 - 1445382

AN - SCOPUS:0026669893

SN - 0158-5231

VL - 28

SP - 1

EP - 9

JO - Biochemistry International

JF - Biochemistry International

IS - 1

ER -

Characterization of bovine heparin-binding neurotrophic factor (HBNF): Assignment of disulfide bonds

Abstract

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