Characterization of a specific antibody to the rat angiotensin II AT1 receptor

Michelle E. Giles, Ross T. Fernley, Yoshio Nakamura, Ingrid Moeller, G. Peter Aldred, Tania Ferraro, Jennifer D. Penschow, Michael J. McKinley, Brian J. Oldfield

Research output: Contribution to journalArticleResearchpeer-review

63 Citations (Scopus)

Abstract

We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunogold-labeled receptor on the surfaces of zona glomerulosa cells in the adrenal cortex, (b) the specific labeling of Chinese hamster ovarian (CHO) cells transfected with AT1 receptors, (c) the identification of a specific band on Western blots, (d) the immunocytochemical co-localization of angiotensin receptors on neurons in the lamina terminalis of the brain shown to be responsive to circulating angiotensin II, as shown by the expression of c-fos, and (e) the correlation between the expression of the mRNA of the AT1 receptor and AT1 receptor immunoreactivity.

Original languageEnglish
Pages (from-to)507-515
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume47
Issue number4
DOIs
Publication statusPublished - 1 Jan 1999
Externally publishedYes

Keywords

  • Angiotensin II
  • AT receptor
  • Lamina terminalis

Cite this