Characterization of β-adrenoceptor mediated smooth muscle relaxation and the detection of mRNA for β1-, β2- and β3-adrenoceptors in rat ileum

S. J. Roberts, M. Papaioannou, B. A. Evans, R. J. Summers

Research output: Contribution to journalArticleResearchpeer-review

Abstract

1. Functional and molecular approaches were used to characterize the β-AR subtypes mediating relaxation of rat ileal smooth muscle. 2. In functional studies, (-)-isoprenaline relaxation was unchanged by CGP20712A (β1-AR antagonist) or ICI118551 (β2-AR antagonist) but shifted by propranolol (pK(B) = 6.69). (±)-Cyanopindolol, CGP12177 and ICID7114 did not cause relaxation but antagonized (-)-isoprenaline relaxation. 3. BRL37344 (β3-AR agonist) caused biphasic relaxation. The high affinity component was shifted with low affinity by propranolol, (±)-cyanopindolol, tertatolol and alprenolol. CL316243 (β3-AR agonist) relaxation was unaffected by CGP20712A or ICI118551 but blocked by SR58894A (β3-AR antagonist; pA2 = 7.80). Enhanced relaxation after exposure to forskolin and pertussis toxin showed that β3-AR relaxation can be altered by manipulation of components of the adenylate cyclase signalling pathway. 4. The β1-AR agonist RO363 relaxed the ileum (pEC50 = 6.18) and was blocked by CGP20712A. Relaxation by the β2-AR agonist zinterol (pEC50 = 5.71) was blocked by SR58894A but not by ICI118551. 5. In rat ileum, β1-, β2- and β3-AR mRNA was detected. Comparison of tissues showed that β3-AR mRNA expression was greatest in WAT > colon = ileum > cerebral cortex > soleus; β1-AR mRNA was most abundant in cerebral cortex > WAT > ileum = colon > soleus; β2-AR mRNA was expressed in soleus > WAT > ileum = colon > cerebral cortex. 6. These results show that β3-ARs are the predominant β-AR subtype mediating rat ileal relaxation while β1-ARs may produce a small relaxation. The β2-AR agonist zinterol produces relaxation through β3-ARs and there was no evidence for the involvement of β2-ARs in relaxation despite the detection of β2-AR mRNA.

Original languageEnglish
Pages (from-to)949-961
Number of pages13
JournalBritish Journal of Pharmacology
Volume127
Issue number4
DOIs
Publication statusPublished - 6 Jul 1999

Keywords

  • β-adrenoceptors
  • Gastrointestinal smooth muscle
  • Messenger RNA
  • Rat ileum
  • Relaxation

Cite this

@article{795447ce88a848c9892040505dfca8de,
title = "Characterization of β-adrenoceptor mediated smooth muscle relaxation and the detection of mRNA for β1-, β2- and β3-adrenoceptors in rat ileum",
abstract = "1. Functional and molecular approaches were used to characterize the β-AR subtypes mediating relaxation of rat ileal smooth muscle. 2. In functional studies, (-)-isoprenaline relaxation was unchanged by CGP20712A (β1-AR antagonist) or ICI118551 (β2-AR antagonist) but shifted by propranolol (pK(B) = 6.69). (±)-Cyanopindolol, CGP12177 and ICID7114 did not cause relaxation but antagonized (-)-isoprenaline relaxation. 3. BRL37344 (β3-AR agonist) caused biphasic relaxation. The high affinity component was shifted with low affinity by propranolol, (±)-cyanopindolol, tertatolol and alprenolol. CL316243 (β3-AR agonist) relaxation was unaffected by CGP20712A or ICI118551 but blocked by SR58894A (β3-AR antagonist; pA2 = 7.80). Enhanced relaxation after exposure to forskolin and pertussis toxin showed that β3-AR relaxation can be altered by manipulation of components of the adenylate cyclase signalling pathway. 4. The β1-AR agonist RO363 relaxed the ileum (pEC50 = 6.18) and was blocked by CGP20712A. Relaxation by the β2-AR agonist zinterol (pEC50 = 5.71) was blocked by SR58894A but not by ICI118551. 5. In rat ileum, β1-, β2- and β3-AR mRNA was detected. Comparison of tissues showed that β3-AR mRNA expression was greatest in WAT > colon = ileum > cerebral cortex > soleus; β1-AR mRNA was most abundant in cerebral cortex > WAT > ileum = colon > soleus; β2-AR mRNA was expressed in soleus > WAT > ileum = colon > cerebral cortex. 6. These results show that β3-ARs are the predominant β-AR subtype mediating rat ileal relaxation while β1-ARs may produce a small relaxation. The β2-AR agonist zinterol produces relaxation through β3-ARs and there was no evidence for the involvement of β2-ARs in relaxation despite the detection of β2-AR mRNA.",
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year = "1999",
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Characterization of β-adrenoceptor mediated smooth muscle relaxation and the detection of mRNA for β1-, β2- and β3-adrenoceptors in rat ileum. / Roberts, S. J.; Papaioannou, M.; Evans, B. A.; Summers, R. J.

In: British Journal of Pharmacology, Vol. 127, No. 4, 06.07.1999, p. 949-961.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Characterization of β-adrenoceptor mediated smooth muscle relaxation and the detection of mRNA for β1-, β2- and β3-adrenoceptors in rat ileum

AU - Roberts, S. J.

AU - Papaioannou, M.

AU - Evans, B. A.

AU - Summers, R. J.

PY - 1999/7/6

Y1 - 1999/7/6

N2 - 1. Functional and molecular approaches were used to characterize the β-AR subtypes mediating relaxation of rat ileal smooth muscle. 2. In functional studies, (-)-isoprenaline relaxation was unchanged by CGP20712A (β1-AR antagonist) or ICI118551 (β2-AR antagonist) but shifted by propranolol (pK(B) = 6.69). (±)-Cyanopindolol, CGP12177 and ICID7114 did not cause relaxation but antagonized (-)-isoprenaline relaxation. 3. BRL37344 (β3-AR agonist) caused biphasic relaxation. The high affinity component was shifted with low affinity by propranolol, (±)-cyanopindolol, tertatolol and alprenolol. CL316243 (β3-AR agonist) relaxation was unaffected by CGP20712A or ICI118551 but blocked by SR58894A (β3-AR antagonist; pA2 = 7.80). Enhanced relaxation after exposure to forskolin and pertussis toxin showed that β3-AR relaxation can be altered by manipulation of components of the adenylate cyclase signalling pathway. 4. The β1-AR agonist RO363 relaxed the ileum (pEC50 = 6.18) and was blocked by CGP20712A. Relaxation by the β2-AR agonist zinterol (pEC50 = 5.71) was blocked by SR58894A but not by ICI118551. 5. In rat ileum, β1-, β2- and β3-AR mRNA was detected. Comparison of tissues showed that β3-AR mRNA expression was greatest in WAT > colon = ileum > cerebral cortex > soleus; β1-AR mRNA was most abundant in cerebral cortex > WAT > ileum = colon > soleus; β2-AR mRNA was expressed in soleus > WAT > ileum = colon > cerebral cortex. 6. These results show that β3-ARs are the predominant β-AR subtype mediating rat ileal relaxation while β1-ARs may produce a small relaxation. The β2-AR agonist zinterol produces relaxation through β3-ARs and there was no evidence for the involvement of β2-ARs in relaxation despite the detection of β2-AR mRNA.

AB - 1. Functional and molecular approaches were used to characterize the β-AR subtypes mediating relaxation of rat ileal smooth muscle. 2. In functional studies, (-)-isoprenaline relaxation was unchanged by CGP20712A (β1-AR antagonist) or ICI118551 (β2-AR antagonist) but shifted by propranolol (pK(B) = 6.69). (±)-Cyanopindolol, CGP12177 and ICID7114 did not cause relaxation but antagonized (-)-isoprenaline relaxation. 3. BRL37344 (β3-AR agonist) caused biphasic relaxation. The high affinity component was shifted with low affinity by propranolol, (±)-cyanopindolol, tertatolol and alprenolol. CL316243 (β3-AR agonist) relaxation was unaffected by CGP20712A or ICI118551 but blocked by SR58894A (β3-AR antagonist; pA2 = 7.80). Enhanced relaxation after exposure to forskolin and pertussis toxin showed that β3-AR relaxation can be altered by manipulation of components of the adenylate cyclase signalling pathway. 4. The β1-AR agonist RO363 relaxed the ileum (pEC50 = 6.18) and was blocked by CGP20712A. Relaxation by the β2-AR agonist zinterol (pEC50 = 5.71) was blocked by SR58894A but not by ICI118551. 5. In rat ileum, β1-, β2- and β3-AR mRNA was detected. Comparison of tissues showed that β3-AR mRNA expression was greatest in WAT > colon = ileum > cerebral cortex > soleus; β1-AR mRNA was most abundant in cerebral cortex > WAT > ileum = colon > soleus; β2-AR mRNA was expressed in soleus > WAT > ileum = colon > cerebral cortex. 6. These results show that β3-ARs are the predominant β-AR subtype mediating rat ileal relaxation while β1-ARs may produce a small relaxation. The β2-AR agonist zinterol produces relaxation through β3-ARs and there was no evidence for the involvement of β2-ARs in relaxation despite the detection of β2-AR mRNA.

KW - β-adrenoceptors

KW - Gastrointestinal smooth muscle

KW - Messenger RNA

KW - Rat ileum

KW - Relaxation

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U2 - 10.1038/sj.bjp.0702605

DO - 10.1038/sj.bjp.0702605

M3 - Article

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SP - 949

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JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 1476-5381

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