Characterization and Purification of Mouse Mucosal-Associated Invariant T (MAIT) Cells

Zhenjun Chen, Huimeng Wang, Criselle D'Souza, Hui Fern Koay, Bronwyn Meehan, Zhe Zhao, Troi Pediongco, Mai Shi, Tianyuan Zhu, Bingjie Wang, Lars Kjer-Nielsen, Sidonia B.G. Eckle, Jamie Rossjohn, David P. Fairlie, Dale I. Godfrey, Richard A. Strugnell, James McCluskey, Alexandra J. Corbett

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3 Citations (Scopus)

Abstract

This unit describes the utility of various mouse models of infection and immunization for studying mucosal-associated invariant T (MAIT) cell immunity: MAIT cells can be isolated from the lungs (or from other tissues/organs) and then identified and characterized by flow cytometry using MR1 tetramers in combination with a range of antibodies. The response kinetics, cytokine profiles, and functional differentiation of lung MAIT cells are studied following infection with the bacterial pathogen Legionella longbeachae or Salmonella enterica Typhimurium or immunization with synthetic MAIT cell antigen plus Toll-like receptor agonist. MAIT cells enriched or expanded during the process can be used for further studies. A step-by-step protocol is provided for MAIT cell sorting and adoptive transfer. Mice can then be challenged and MAIT cells tracked and further examined.

Original languageEnglish
Article numbere89
Number of pages30
JournalCurrent Protocols in Immunology
Volume127
Issue number1
DOIs
Publication statusPublished - Dec 2019

Keywords

  • flow cytometry
  • mouse models
  • MR1 tetramers
  • mucosal-associated invariant T (MAIT) cells
  • respiratory infection

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