Characterization and expression of an Fcγ receptor cDNA cloned from rat natural killer cells

Donna L. Zeger, P. Mark Hogarth, Duane W. Sears

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A cDNA clone for an IgG-binding Fc receptor, rtFcγRα, of the rat natural killer cell line CRNK-16 is characterized here. This clone encodes an Fcγ receptor as shown by the ability of cDNA-transfected COS cells to rosette IgG-coated sheep erythrocytes. The rtFcγRα is exceptionally homologous to the mouse moFcγRα, with 77% protein sequence identity and 71% nucleic acid identity overall. The transmembrane region of the rtFcγRα contains the sequence Leu-Phe-Ala-Val-Asp-Thr-Gly-Leu, which is present in the membrane sequences of four other Fc receptors including mouse FcγRα, human FcγRIII-2, and the FcεRα subunits of the rat and human high-affinity IgE-binding receptors. Also, the rtFcγRα cytoplasmic domain exhibits specific homology to other receptors derived from natural killer cells, human FcγRIII-2 and mouse FcγRα. However, the rtFcγRαr cDNA clone is complementary to at least two different-sized mRNAs expressed by CRNK-16 cells, contrasting the single FcγR-related mRNA species expressed by human and mouse natural killer cells. These rat mRNAs are homologous to both the 5′ and the 3′ end of the cDNA clone, suggesting that they may be (i) splice variants of one transcript or (ii) products of different but highly related genes.

Original languageEnglish
Pages (from-to)3425-3429
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number9
Publication statusPublished - 1 Jan 1990
Externally publishedYes


  • Antibody-dependent cell-mediated cytotoxicity
  • IgG receptor
  • Rat CD16

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