Characterization and de novo sequencing of snow crab tropomyosin enzymatic peptides by both electrospary ionization and matrix-assisted laser desorption ionization QqToF tandemmass spectrometry

Anas Abdel Rahman, Andreas L Lopata, Robyn O'Hehir, John Robinson, Joseph Banoub, Robert Helleur

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26 Citations (Scopus)

Abstract

In the present study, the snow crab TM was isolated and purified. For studying different isoforms of TM, tissues were obtained from claw and leg and the extracted proteins were separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) to yield homogeneous bands. Purified TM was used in western blot analysis to study the reactivity of the TM with specific reactivity of monoclonal and polyclonal antibodies as well as serum of patients with crustacean allergy.
Original languageEnglish
Pages (from-to)372 - 381
Number of pages10
JournalJournal of Mass Spectrometry
Volume45
Issue number4
DOIs
Publication statusPublished - 2010

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